Diagnostic ion filtering targeted screening and isolation of anti‐inflammatory iridoid glycosides from Hedyotis diffusa. Issue 13 (26th May 2021)

• Record Type: Journal Article
• Authors: Shi, Fangying; Tong, Chaoying; He, Chengxin; Shi, Shuyun; Cao, Yuanxin; Wei, Qisheng
• Is Part Of: Journal of separation science. Volume 44:Issue 13(2021)
• Page Start: 2612
• Page End: 2619
• Publication Date: 2021-05-26
• Subjects: anti‐inflammatory -- diagnostic ion filtering -- Hedyotis diffusa -- high‐speed counter current chromatography -- iridoid glycoside; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.202100074
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: Efficient and targeted screening and isolation of bioactive compounds from complex natural products is still a challenging work. Herein, diagnostic ion filtering based high‐performance liquid chromatography–quadrupole time‐of‐flight−tandem mass spectrometry was firstly developed to screen six main iridoid glycosides from Hedyotis diffusa . Then, online extraction‒high‐speed counter current chromatography was proposed for targeted enrichment and preparative isolation using ethyl acetate/ n ‐butanol/water (4.5:0.5:5, v/v/v) as solvent system. After that, Sephadex LH‐20 column chromatography using methanol as solvent system was selected for further purification of six iridoid glycosides with purities over 98%. They were finally identified as monotropein, desacetylasperuloside acid, asperuloside, 6‐ O ‐( Z )‐ p ‐coumaroyl scandoside methyl ester, 6‐ O ‐( Z )‐feruloyl scandoside methyl ester, and 6‐ O ‐( E )‐ p ‐coumaroyl scandoside methyl ester. And their anti‐inflammatory activities were evaluated and confirmed by lipopolysaccharide activated RAW 264.7 macrophages. Obviously, the results provide a scientific basis for the potential applications of H. diffusa, and the developed methodology is efficient and reliable for targeted screening and isolation of bioactive compounds from natural products.