Bridelia ferruginea Produces Antineuroinflammatory Activity through Inhibition of Nuclear Factor-kappa B and p38 MAPK Signalling. (18th December 2012)

• Record Type: Journal Article
• Authors: Olajide, Olumayokun A.; Aderogba, Mutalib A.; Okorji, Uchechukwu P.; Fiebich, Bernd L.
• Other Names: Li Shao Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2012(2012)
• Publication Date: 2012-12-18
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2012/546873
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Bridelia ferruginea is commonly used in traditional African medicine (TAM) for treating various inflammatory conditions. Extracts from the plant have been shown to exhibit anti-inflammatory property in a number of in vivo models. In this study the influence of B. ferruginea (BFE) on the production of PGE2, nitrite, and proinflammatory cytokines from LPS-stimulated BV-2 microglia was investigated. The effects of BFE on cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein expressions were evaluated in LPS-activated rat primary microglia. The roles of NF- κ B and MAPK signalling in the actions of BFE were also investigated. BFE (25–200 μ g) inhibited the production of PGE2, nitrite, tumour necrosis factor- α (TNF α ), and interleukin-6 (IL-6) as well as COX-2 and iNOS protein expressions in LPS-activated microglial cells. Further studies to elucidate the mechanism of anti-inflammatory action of BFE revealed interference with nuclear translocation of NF- κ Bp65 through mechanisms involving inhibition of I κ B degradation. BFE prevented phosphorylation of p38, but not p42/44 or JNK MAPK. It is suggested that Bridelia ferruginea produces anti-inflammatory action through mechanisms involving p38 MAPK and NF- κ B signalling.