TRNAIniCAT inhibits proliferation and promotes apoptosis of laryngeal squamous cell carcinoma cells. Issue 7 (28th May 2021)

• Record Type: Journal Article
• Authors: Li, Jun; Shen, Zhisen; Luo, Lin; Ye, Dong; Deng, Hongxia; Gu, Shanshan; Zhou, Chongchang
• Is Part Of: Journal of clinical laboratory analysis. Volume 35:Issue 7(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-28
• Subjects: biomarkers -- laryngeal squamous cell carcinoma -- tRNA -- tRNA expression profile -- tRNAIniCAT; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.23821
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Background: Laryngeal squamous cell carcinoma (LSCC) brings a heavy blow to the patient's voice. Transfer RNA (tRNA) is a common RNA, the roles of tRNAs in LSCC are largely unknown. Methods: The tRNA expression profile in LSCC tissues and adjacent normal tissues was measured by a tRNA qRT‐PCR array. The expression level of tRNA Ini CAT in LSCC tissues and plasmas was detected by qRT‐PCR. The receiver operating characteristic (ROC) curve was established. tRNA Ini CAT was upregulated by a lentivirus vector in the LSCC cell line. Moreover, tRNA Ini CAT was upregulated in LSCC xenograft nude mouse model and the xenografts were used for pathological analysis and transmission electron microscope (TEM) observation. Results: The top 10 upregulated tRNAs were tRNA Lys CTT ‐1, tRNA Leu TAA, tRNA Phe GAA, tRNA Leu CAG, tRNA Tyr ATA, tRNA Met CAT, tRNA Tyr GTA ‐1, tRNA Thr CGT, tRNA Tyr GTA ‐2, tRNA Ala AGC ; and the top 10 downregulated tRNAs were tRNA Ini CAT, mt‐tRNA Glu TTC, tRNA Val CAC ‐3, mt‐tRNA Trp TCA, mt‐tRNA Tyr GTA, mt‐tRNA Lys TTT, mt‐tRNA Thr TGT, mt‐tRNA Asp GTC, mt‐tRNA Asn GTT, mt‐tRNA Pro TGG . tRNA Ini CAT was downregulated in LSCC tissues and plasma. The area under the ROC curve (AUC) in LSCC tissues and the plasma of patients with LSCC was 0.717 and 0.808, respectively. tRNA Ini CAT inhibited LSCC cell proliferation and promoted apoptosis. The in vivo results showed that tRNA Ini CAT inhibited the growth of the xenografts and promoted apoptosis. Conclusions: This is the first study to provide tRNA expression profiles for LSCC tissues. tRNA Ini CAT may be used as a new biomarker for the early diagnosis of LSCC. tRNA Ini CAT inhibits cell proliferation and promotes apoptosis in vitro and in vivo . Abstract : tRNAIniCAT inhibited the proliferation and induced the apoptosis of LSCC cells in vivo. A: Nude mouse model of xenograft. (a) NC. (b) Low dose. (c) High dose. B: Tumor weight in high‐dose tRNAIniCAT‐treated groups were significantly lower than the NC group. * p <0.05. C: There was no significant difference in tumor volume between each group. D: There was no significant difference in the weight of mice between each group. E: Pathological examination showed that tumor necrosis in the high‐dose treatment group was markedly increased compared with that observed in the NC group (magnification ×100). (a) NC. (b) Low dose. (c) High dose. F: TEM results showing that autophagy and apoptosis were more observed in the treatment group (b and c) (magnification ×20, 000). (a) NC. (b) Low dose. (c) High dose.