UV-C Exposure Induces an Apoptosis-Like Process in Euglena gracilis. (17th January 2013)

• Record Type: Journal Article
• Authors: Bumbulis, Michael J.; Balog, Brian M.
• Other Names: Colanzi A. Academic Editor.; Yamaguchi M. Academic Editor.
• Is Part Of: ISRN cell biology. Volume 2013(2013)
• Publication Date: 2013-01-17
• Subjects: Cytology -- Periodicals; Cells; Cell Biology; Cells; Cytological Techniques; Biology; Cells; Cytology; Periodicals; Electronic journals; 571.6
• DOI: 10.1155/2013/869216
• Languages: English
• ISSNs: 2090-7370

Abstract

Abstract : Euglena gracilis is a unicellular, free-living flagellate that inhabits various freshwater environments. Our research shows that exposure to UV-C light can trigger some form of programmed cell death. Cells exposed to UV-C light underwent delayed changes that were strongly reminiscent of apoptosis in mammalian cells, including cell shrinkage and DNA fragmentation that produced the characteristic ladder pattern commonly seen with apoptosis. DNA fragmentation could be inhibited by pretreatment with Z-VAD-FMK and also independently induced by exposure to staurosporine. In addition, Euglena possess proteins that cross-reacted with antibodies raised against human caspases 3 and 9. Given that Euglena are extremely easy to culture and represent a lineage positioned near the base of the eukaryotic tree, they will be an excellent model system for comparative analyses with apoptotic-like death processes in other eukaryotic microbes.