Intracellular expression of granzymes A, B, K and M in blood lymphocyte subsets of critically ill patients with or without sepsis. (27th May 2021)

• Record Type: Journal Article
• Authors: García‐Laorden, M. Isabel; Hoogendijk, Arie J.; Wiewel, Maryse A.; van Vught, Lonneke A.; Schultz, Marcus J.; Bovenschen, Niels; de Vos, Alex F.; van der Poll, Tom
• Is Part Of: Clinical and experimental immunology. Volume 205:Number 2(2021)
• Page Start: 222
• Page End: 231
• Publication Date: 2021-05-27
• Subjects: granzymes -- infection -- inflammation -- sepsis; Immunopathology -- Periodicals; 616.079
• DOI: 10.1111/cei.13601
• Languages: English
• ISSNs: 0009-9104

Abstract

Summary: Sepsis is a complex syndrome related to an infection‐induced exaggerated inflammatory response, which is associated with a high mortality. Granzymes (Gzm) are proteases mainly found in cytotoxic lymphocytes that not only have a role in target cell death, but also as mediators of infection and inflammation. In this study we sought to analyse the intracellular expression of GzmA, B, M and K by flow cytometry in diverse blood lymphocyte populations from 22 sepsis patients, 12 non‐infected intensive care unit (ICU) patients and 32 healthy controls. Additionally, we measured GzmA and B plasma levels. Both groups of patients presented decreased percentage of natural killer (NK) cells expressing GzmA, B and M relative to healthy controls, while sepsis patients showed an increased proportion of CD8 + T cells expressing GzmB compared to controls. Expression of GzmK remained relatively unaltered between groups. Extracellular levels of GzmB were increased in non‐infected ICU patients relative to sepsis patients and healthy controls. Our results show differential alterations in intracellular expression of Gzm in sepsis patients and non‐infected critically ill patients compared to healthy individuals depending on the lymphocyte population and on the Gzm. Abstract : This study reports differential alterations in intracellular expression of granzymes A, B and M in sepsis patients and non‐infected critically ill patients compared to healthy individuals depending on the lymphocyte population and on the granzyme subtype. Expression of granzyme K remained relatively unaltered between groups