Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice. (29th December 2012)

• Record Type: Journal Article
• Authors: Chou, Shen-Chieh; Chiu, Yung-Jia; Chen, Chao-Jung; Lin, Ying-Chih; Wu, Chung-Hao; Chao, Chien-Ti; Chang, Ching-Wen; Peng, Wen-Huang
• Other Names: Shirwaikar Annie Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2012(2012)
• Publication Date: 2012-12-29
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2012/138954
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : The aim of this study was to investigate the possible analgesic and anti-inflammatory mechanisms of the ethanolic extract of A. morrisonensis Hayata (AM EtOH ). Two models were employed for evaluation of the analgesic effects: acetic acid-induced writhing response and formalin-induced paw licking. The results demonstrated that AM EtOH decreased writhing response for both the acetic acid assay and the licking time in the formalin test. The anti-inflammatory effect was evaluated by paw edema of mice induced by λ -carrageenan. AM EtOH significantly decreased induced paw edema three to four hours after λ -carrageenan injection. Additionally, the results indicated that the anti-inflammatory mechanism of AM EtOH may be due to the declined levels of nitric oxide (NO) and malondialdehyde (MDA) in the edematous paw. Furthermore, AM EtOH decreased the tumor necrosis factor-alpha (TNF- α ) and interleukin-6 (IL-6) levels, leading to the reduction of prostaglandins and subsequently alleviated edema. Isolation and purification of the AM EtOH extract determined p -hydroxyacetophenone to be a major component at 130 mg/g of extract. No mortality was observed in the acute toxicity test given at the dose of 10 g/kg. This study demonstrated the possible mechanisms for the analgesic and anti-inflammatory effects of AM EtOH for mice and provided evidence for the ethnobotanical uses of A. morrisonensis in treating inflammatory diseases.