Comparative Study of Corneal Endothelial Cell Damage after Femtosecond Laser Assisted Deep Stromal Dissection. (10th July 2014)

• Record Type: Journal Article
• Authors: Liu, Ting; Zhang, Jingjing; Sun, Dapeng; Sui, Wenjie; Zhang, Yangyang; Li, Dongfang; Chen, Zhaoli; Gao, Hua
• Other Names: Costagliola Ciro Academic Editor.
• Is Part Of: BioMed research international. Volume 2014(2014)
• Publication Date: 2014-07-10
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2014/731565
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Purpose. To find a relatively safe designed stromal bed thickness to avoid endothelial damage for lamellar keratoplasty with an Allegretto Wavelight FS200 femtosecond laser. Methods. Twelve rabbits were randomly divided into 50 μ m and 150 μ m groups according to the anticipated residue stromal bed thickness preparation with a femtosecond laser. Six rabbits without laser cutting were used as a control group. Central endothelial images were analyzed with in vivo confocal microscopy and scanning electron microscopy. The apoptosis of endothelium was evaluated with Hoechst 33342 staining and a TUNEL assay. Results. The endothelium of the 50 μ m group had extensive injuries upon in vivo confocal and scanning electron microscopic observation, and minor injuries were observed in the 150 μ m group. Moreover, more apoptotic cells were observed in the 50 μ m group. Conclusions. When using a FS200 femtosecond laser assisted anterior lamellar keratoplasty, there was minor endothelium damage with a 150 μ m stromal bed, and a more than 150 μ m thickness stromal bed design may prevent the damage of corneal endothelium.