Development and Validation of RP-HPLC Method for Azilsartan Medoxomil Potassium Quantitation in Human Plasma by Solid Phase Extraction Procedure. (14th September 2013)

• Record Type: Journal Article
• Authors: Vekariya, Paras P.; Joshi, Hitendra S.
• Other Names: Gerothanassis I. P. Academic Editor.; Liu B. Academic Editor.; Pandey S. Academic Editor.
• Is Part Of: ISRN spectroscopy. Volume 2013(2013)
• Publication Date: 2013-09-14
• Subjects: Spectrum analysis -- Periodicals; Spectrum analysis; Periodicals; Electronic journals; 543.5
• DOI: 10.1155/2013/572170
• Languages: English
• ISSNs: 2090-8776

Abstract

Abstract : Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated using solid phase extraction (SPE) technique for the determination of Azilsartan Medoxomil Potassium (AMP) in human plasma; detection was carried out by photo diode array detector. Chromatographic separation of the analyte AMP was achieved within 7.5 min by Waters symmetry C18 (4.6 × 250 mm, 5 µm) column, mobile phase was 25 mM ammonium acetate buffer (pH 5.5): acetonitrile 55 : 45 v/v, flow rate was 1.0 mL/min, and the detection was carried out at 254 nm. Calibration curve was linear ( r 2 > 0.9985) in the range of 1.0–9.0 µg/mL, limit of detection (LOD) and limit of quantitation (LOQ) were 0.150 µg/mL and 0.400 µg/mL, respectively, and intra- and interday deviations were between 1.53–8.41% and 1.78–4.59%, respectively. The overall mean recovery of AMP was 92.35%. No any endogenous constituents were found to interfere at retention time of the analyte. This new RP-HPLC method was successfully validated and may be applied to conduct bioavailability and bioequivalence studies of AMP.