A Validated LC Method for Separation and Determination of Tetralone-4-O-β-D-Glucopyranoside and 4-Hydroxy-α-Tetralone in Ammannia multiflora. (20th May 2012)

• Record Type: Journal Article
• Authors: Upadhyay, Harish C.; Verma, Ram K.; Srivastava, Santosh K.
• Other Names: Ozkan Sibel A. Academic Editor.
• Is Part Of: Chromatography research international. Volume 2012(2012)
• Publication Date: 2012-05-20
• Subjects: Chromatographic analysis -- Periodicals; Chromatographic analysis -- Research -- Periodicals; Chromatographic analysis; Chromatographic analysis -- Research; Periodicals
• DOI: 10.1155/2012/162302
• Languages: English
• ISSNs: 2090-3502

Abstract

Abstract : A rapid, sensitive, and reproducible RP-HPLC method was developed for the determination of two constituents in Ammannia multiflora, namely, tetralone-4-O-β-D-glucopyranoside (1) and 4-hydroxy-α-tetralone (2) . The samples were separated on a Spherisorb ODS2 column (250 × 4.6 mm, i.d., 10 μm) and binary elution of water and methanol (2 : 3) with flow rate of 0.8 mL/min at λ 254 nm. The LOD and LOQ were found 0.05 and 0.18 μg/mL for compound 1 and 0.06 and 0.18 μg/mL for compound 2, respectively. All calibration curves showed good linearity (r 2 > 0.999 ) within test ranges for both the analytes. The RSD values for intra-and inter-day precisions were less than 1.1%. The successful application of the developed method on five different samples revealed an average 0.0206% and 0.7636% (w/w) of compounds 1 and 2, respectively in A. multiflora indicating that the developed LC assay method may be readily utilized as a quality control method for the plant.