Vouchering of Forensically Important Fly Specimens by Nondestructive DNA Extraction. (19th December 2012)

• Record Type: Journal Article
• Authors: Kim, Seong Yoon; Park, Seong Hwan; Piao, Huguo; Chung, Ukhee; Ko, Kwang Soo; Hwang, Juck-Joon
• Other Names: Andrade Filho J. D. Academic Editor.; Kehr A. I. Academic Editor.; Klimaszewski J. Academic Editor.
• Is Part Of: ISRN entomology. Volume 2013(2013)
• Publication Date: 2012-12-19
• Subjects: Entomology -- Periodicals; Entomology; Electronic journals; Periodicals; Electronic journals; 595.7
• DOI: 10.1155/2013/286182
• Languages: English
• ISSNs: 2314-5447

Abstract

Abstract : DNA extraction frequently requires destruction of whole samples. However, when the sample is very rare or has taxonomic importance, nondestructive DNA extraction is required for preservation of voucher specimens. In the case of arthropod specimens, minor anatomical structures such as a single leg or a single wing are often sacrificed instead of the whole body for DNA extraction. In an attempt to save the entire anatomical structure of specimens, several authors tried to brew the whole specimen in a lysis buffer and to extract DNA from the "soup." We applied this nondestructive DNA extraction technique to a forensically important blowfly species, Phaenicia sericata . With nondestructive DNA extraction, a satisfactory quantity and quality of DNA for PCR amplification was obtained with only minimal anatomical disruptions that do not alter the morphologic identification. This nondestructive method may be applicable to DNA extraction of rare samples as well as vouchering of regular fly samples.