Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2. Issue 14 (8th June 2021)

• Record Type: Journal Article
• Authors: Yin, Kun; Ding, Xiong; Li, Ziyue; Sfeir, Maroun M.; Ballesteros, Enrique; Liu, Changchun
• Is Part Of: Lab on a chip. Volume 21:Issue 14(2021)
• Page Start: 2730
• Page End: 2737
• Publication Date: 2021-06-08
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/d1lc00293g
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : Autonomous lab-on-paper platform for simple, rapid, low-cost, and multiplex gene diagnosis of SARS-CoV-2 in clinical samples. Abstract : The COVID-19 pandemic, caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a public health emergency and widely spread around the world. Rapid, accurate and early diagnosis of COVID-19 infection plays a crucial role in breaking this pandemic. However, the detection accuracy is limited for current single-gene diagnosis of SARS-CoV-2. Herein, we develop an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2 by combining reverse transcription recombinase polymerase amplification (RT-RPA) and CRISPR–Cas12a detection. The autonomous lab-on-paper is capable of simultaneously detecting nucleoprotein (N) gene and spike (S) gene of SARS-CoV-2 virus as well as human housekeeping RNAse P gene (an internal control) in a single clinical sample. With the developed platform, 10 2 copies viral RNA per test can be detected within one hour. Also, the lab-on-paper platform has been used to detect 21 swab clinical samples and obtains a comparable performance to the conventional RT-PCR method. Thus, the developed lab-on-paper platform holds great potential for rapid, sensitive, reliable, multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings.