Electrochemical Monitoring of ROS/RNS Homeostasis Within Individual Phagolysosomes Inside Single Macrophages. (2nd May 2019)

• Record Type: Journal Article
• Authors: Zhang, Xin‐Wei; Oleinick, Alexander; Jiang, Hong; Liao, Quan‐Lan; Qiu, Quan‐Fa; Svir, Irina; Liu, Yan‐Ling; Amatore, Christian; Huang, Wei‐Hua
• Is Part Of: Angewandte Chemie. Volume 131:Number 23(2019)
• Page Start: 7835
• Page End: 7838
• Publication Date: 2019-05-02
• Subjects: Homöostase -- Nanoelektroden-Amperometrie -- Phagolysosomen -- ROS/RNS-Produktion; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201902734
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: The existence of a homeostatic mechanism regulating reactive oxygen/nitrogen species (ROS/RNS) amounts inside phagolysosomes has been invoked to account for the efficiency of this process but could not be unambiguously documented. Now, intracellular electrochemical analysis with platinized nanowire electrodes (Pt‐NWEs) allowed monitoring ROS/RNS effluxes with sub‐millisecond resolution from individual phagolysosomes impacting onto the electrode inserted inside a living macrophage. This shows for the first time that the consumption of ROS/RNS by their oxidation at the nanoelectrode surface stimulates the production of significant ROS/RNS amounts inside phagolysosomes. These results establish the existence of the long‐postulated ROS/RNS homeostasis and allows its kinetics and efficiency to be quantified. ROS/RNS concentrations may then be maintained at sufficiently high levels for sustaining proper pathogen digestion rates without endangering the macrophage internal structures. Abstract : Amperometrie mit Nanodrahtelektroden wurde verwendet, um festzustellen, dass der Verbrauch von ROS/RNS in einem Phagolysosom zu einer ROS/RNS‐Produktion führt. Dies validiert den lang gesuchten homöostatischen ROS/RNS‐Mechanismus, von dem angenommen wurde, dass er den intravesikulären ROS/RNS‐Gehalt während des Pathogenverdaus reguliert.