Nanoengineering Hybrid Supramolecular Multilayered Biomaterials Using Polysaccharides and Self‐Assembling Peptide Amphiphiles. (15th February 2017)

• Record Type: Journal Article
• Authors: Borges, João; Sousa, Maria P.; Cinar, Goksu; Caridade, Sofia G.; Guler, Mustafa O.; Mano, João F.
• Is Part Of: Advanced functional materials. Volume 27:Number 17(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-02-15
• Subjects: layer‐by‐layer assembly -- peptide amphiphiles -- polysaccharides -- self‐assembly -- supramolecular nanostructured multilayered biomaterials; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.201605122
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract : Developing complex supramolecular biomaterials through highly dynamic and reversible noncovalent interactions has attracted great attention from the scientific community aiming key biomedical and biotechnological applications, including tissue engineering, regenerative medicine, or drug delivery. In this study, the authors report the fabrication of hybrid supramolecular multilayered biomaterials, comprising high‐molecular‐weight biopolymers and oppositely charged low‐molecular‐weight peptide amphiphiles (PAs), through combination of self‐assembly and electrostatically driven layer‐by‐layer (LbL) assembly approach. Alginate, an anionic polysaccharide, is used to trigger the self‐assembling capability of positively charged PA and formation of 1D nanofiber networks. The LbL technology is further used to fabricate supramolecular multilayered biomaterials by repeating the alternate deposition of both molecules. The fabrication process is monitored by quartz crystal microbalance, revealing that both materials can be successfully combined to conceive stable supramolecular systems. The morphological properties of the systems are studied by advanced microscopy techniques, revealing the nanostructured dimensions and 1D nanofibrous network of the assembly formed by the two molecules. Enhanced C2C12 cell adhesion, proliferation, and differentiation are observed on nanostructures having PA as outermost layer. Such supramolecular biomaterials demonstrate to be innovative matrices for cell culture and hold great potential to be used in the near future as promising biomimetic supramolecular nanoplatforms for practical applications. Abstract : The fabrication of hybrid supramolecular nanostructured multilayered biomaterials, comprising high‐molecular‐weight alginate biopolymer and oppositely charged low‐molecular‐weight self‐assembling peptide amphiphile molecule, is reported through the combination of self‐assembly and electrostatically driven layer‐by‐layer assembly approach. Enhanced cell adhesion, proliferation, and differentiation are observed on supramolecular nanostructures having peptide amphiphile as the outermost layer, constituting promising bioinstructive matrices for biomedical and healthcare applications.