A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models. Issue 10 (1st October 2019)

• Record Type: Journal Article
• Authors: Pandard, Justine; Pan, Na; Ebene, Dina H.; Le Saux, Thomas; Ait‐Yahiatène, Eric; Liu, Xiaoqing; Grimaud, Laurence; Buriez, Olivier; Labbé, Eric; Lemaître, Frédéric; Guille‐Collignon, Manon
• Is Part Of: ChemPlusChem. Volume 84:Issue 10(2019)
• Page Start: 1578
• Page End: 1586
• Publication Date: 2019-10-01
• Subjects: electrochemistry -- exocytosis -- fluorescent false neurotransmitters -- indium tin oxide -- photophysics; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/cplu.201900385
• Languages: English
• ISSNs: 2192-6506

Abstract

Abstract: A dual electrofluorescent probe (FFN42) belonging to the fluorescent false neurotransmitter family was rationally designed for investigating cell secretion. This probe, which comprises a coumarin core with one amino and two hydroxy groups, is very promising due to its electroactive and fluorescent properties. The optimal excitation and emission wavelengths (380 nm and 470 nm respectively) make this probe adapted for use in fluorescence microscopy. FFN42 has a quantum yield of 0.18, a molar absorption coefficient of 12000 M −1 cm −1 and pKa values of 5.4 and 6.7 for the hydroxy groups. The electroactivity of FFN42 was evidenced on carbon fiber and ITO electrodes at relatively low oxidation potentials (0.24 V and 0.45 V vs Ag/AgCl respectively). Epifluorescence observations showed that FFN42 accumulated into secretory vesicles of PC12 and N13 cells. Toxicity tests further revealed that FFN42 had no lethal effect on these cells. Amperometric data obtained on carbon fiber electrodes proved that the probe is released by N13 cells. Abstract : Studying secretion : A false fluorescent neurotransmitter (FFN42) was synthesized for investigating exocytosis at the single‐cell level. The photophysical and electrochemical properties of FFN42 are ideal for studying secretion. Biological experiments on secretory cell models (PC12 and BON) were performed by using fluorescence and amperometric techniques. FFN42 was shown to be nontoxic and can be taken up in secretory vesicles and then released after stimulation.