Vendor‐specific microbiome controls both acute and chronic murine lung allograft rejection by altering CD4+Foxp3+ regulatory T cell levels. Issue 10 (2nd August 2019)

• Record Type: Journal Article
• Authors: Guo, Yizhan; Wang, Qing; Li, Dongge; Onyema, Oscar Okwudiri; Mei, Zhongcheng; Manafi, Amir; Banerjee, Anirban; Mahgoub, Bayan; Stoler, Mark H.; Barker, Thomas H.; Wilkes, David S.; Gelman, Andrew E.; Kreisel, Daniel; Krupnick, Alexander Sasha
• Is Part Of: American journal of transplantation. Volume 19:Issue 10(2019)
• Page Start: 2705
• Page End: 2718
• Publication Date: 2019-08-02
• Subjects: animal models: murine -- basic (laboratory) research/science -- bronchiolitis obliterans (BOS) -- immunosuppression/immune modulation -- lung disease: immune/inflammatory -- lung transplantation/pulmonology -- rejection: acute -- rejection: chronic; Transplantation of organs, tissues, etc -- Periodicals; 617.95
• DOI: 10.1111/ajt.15523
• Languages: English
• ISSNs: 1600-6135

Abstract

Abstract : Despite standardized postoperative care, some lung transplant patients suffer multiple episodes of acute and chronic rejection while others avoid graft problems for reasons that are poorly understood. Using an established model of C57BL/10 to C57BL/6 minor antigen mismatched single lung transplantation, we now demonstrate that the recipient microbiota contributes to variability in the alloimmune response. Specifically, mice from the Envigo facility in Frederick, Maryland contain nearly double the number of CD4 + Foxp3 + regulatory T cells (Tregs ) than mice from the Jackson facility in Bar Harbor, Maine or the Envigo facility in Indianapolis, Indiana (18 vs 9 vs 7%). Lung graft recipients from the Maryland facility thus do not develop acute or chronic rejection. Treatment with broad‐spectrum antibiotics decreases Tregs and increases both acute and chronic graft rejection in otherwise tolerant strains of mice. Constitutive depletion of regulatory T cells, using Foxp3‐driven expression of diphtheria toxin receptor, leads to the development of chronic rejection and supports the role of Tregs in both acute and chronic alloimmunity. Taken together, our data demonstrate that the microbiota of certain individuals may contribute to tolerance through Treg ‐dependent mechanisms and challenges the practice of indiscriminate broad‐spectrum antibiotic use in the perioperative period. Abstract : This article demonstrates that vendor‐specific differences in the microbiome can contribute to variability in both acute and chronic rejection by altering the balance of pathogenic T helper 17 cells and CD4+Foxp3+ regulatory T cells. See the editorial by Husson et al on page 2673 .