Sortase A‐mediated modification of the Streptococcus mutans transcriptome and virulence traits. (9th August 2019)

• Record Type: Journal Article
• Authors: Chen, Xuan; Liu, Chengcheng; Peng, Xian; He, Yuanli; Liu, Haixia; Song, Ying; Xiong, Kaixin; Zou, Ling
• Is Part Of: Molecular oral microbiology. Volume 34:Number 5(2019)
• Page Start: 219
• Page End: 233
• Publication Date: 2019-08-09
• Subjects: acid tolerance -- exopolysaccharides production -- Sortase A -- srtA -- Streptococcus mutans -- transcriptome; Mouth -- Microbiology -- Periodicals; Respiratory infections -- Microbiology -- Periodicals; Mouth -- Diseases -- Immunological aspects -- Periodicals; 617.522
• DOI: 10.1111/omi.12266
• Languages: English
• ISSNs: 2041-1006

Abstract

Abstract: Sortase A contributes to adhesion and biofilm formation of Streptococcus mutans by anchoring surface proteins like P1 onto the cell wall, and few other functional characterization has been annotated to this protein and its coding gene srtA . In this study we investigated that whether srtA deletion would affect S. mutans virulence determinants in addition to adhesion and further explored whether these effects were caused due to changes in S. mutans genomic transcription. We used acid‐killing assays, glycolytic rate assessments, and exopolysaccharide (EPS) formation tests to detect whether srtA deletion influenced S. mutans acid tolerance/production and glucan formation. Comparisons between RNA‐sequencing data from both the exponential and stationary phases of UA159 and the srtA ‐deleted strain were made to determine the impact of srtA knockout on S. mutans genomic transcription. Results of our assays indicated that S. mutans aciduricity was enhanced in the srtA deleted strain when bacterial cells were directly subjected to pH 2.8, but the enhancement was repressed when the acid tolerance response was induced in advance. The srtA mutation strain exhibited reduced EPS formation in mature biofilms. SrtA deletion led to pleiotropic changes in the S. mutans transcriptome with a growth phase‐dependent pattern. The affected genes mainly included those involved in aciduricity, carbohydrate transport, and EPS formation. It was concluded that S. mutans srtA exhibited multiple effects on the virulence traits of this pathogen, including acid tolerance and glucan formation, and that these alterations could be partially due to transcriptional changes upon loss of srtA . Abstract : Deletion of srtA disturbed Streptococcus mutans acid tolerance and generated less exopolysaccharides in biofilms. Transcriptome analysis of S. mutans strains indicated that the differential expression of genes involved in aciduricity and glucan formation in response to srtA deletion might be the reason for those observed phenomena.