Fragmentation pathways of deprotonated amide‐sulfonamide CXCR4 inhibitors investigated by ESI‐IT‐MSn, ESI‐Q‐TOF‐MS/MS and DFT calculations. (20th November 2019)

• Record Type: Journal Article
• Authors: Guo, Zili; Jie, Xiaokang; Zhu, Peixi; Sun, Jian; Gu, Jinping; Su, Feng; Bai, Renren; Xie, Yuanyuan
• Is Part Of: Journal of mass spectrometry. Volume 54:Number 11(2019)
• Page Start: 869
• Page End: 877
• Publication Date: 2019-11-20
• Subjects: amide‐sulfonamides -- deuterium–hydrogen exchange -- DFT calculations -- ESI‐QTOF‐MS/MS -- fragmentation pathways; Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.4439
• Languages: English
• ISSNs: 1076-5174

Abstract

Abstract: Amide‐sulfonamides provide a potent anti‐inflammatory scaffold targeting the CXCR4 receptor. A series of novel amide‐sulfonamide derivatives were investigated for their gas‐phase fragmentation behaviors using electrospray ionization ion trap mass spectrometry and quadrupole time‐of‐flight mass spectrometry in negative ion mode. Upon collision‐induced dissociation (CID), deprotonated amide‐sulfonamides mainly underwent either an elimination of the amine to form the sulfonyl anion and amide anion or a benzoylamide derivative to provide sulfonamide anion bearing respective substituent groups. Based on the characteristic fragment ions and the deuterium–hydrogen exchange experiments, three possible fragmentation mechanisms corresponding to ion‐neutral complexes including [sulfonyl anion/amine] complex (INC‐1 ), [sulfonamide anion/benzoylamide derivative] complex (INC‐2 ) and [amide anion/sulfonamide] complex (INC‐3 ), respectively, were proposed. These three ion‐neutral complexes might be produced by the cleavages of S–N and C–N bond from the amide‐sulfonamides, which generated the sulfonyl anion (Route 1), sulfonamide anion (Route 2) and the amide anion (Route 3). DFT calculations suggested that Route 1, which generated the sulfonyl anion (ion c ) is more favorable. In addition, the elimination of SO2 through a three‐membered‐ring transition state followed by the formation of C–N was observed for all the amide‐sulfonamides.