CPF impedes cell cycle re‐entry of quiescent lung cancer cells through transcriptional suppression of FACT and c‐MYC. Issue 3 (20th January 2020)

• Record Type: Journal Article
• Authors: Bi, Ling; Xie, Chanlu; Jiao, Lijing; Jin, Shenyi; Hnit, Su Su Thae; Mu, Yao; Wang, Yilun; Wang, Qian; Ge, Guangbo; Wang, Yaqiao; Zhao, Xiaodong; Shi, Xinglong; Kang, Yani; De Souza, Paul; Liu, Tao; Zhou, Jia; Xu, Ling; Dong, Qihan
• Is Part Of: Journal of cellular and molecular medicine. Volume 24:Issue 3(2020)
• Page Start: 2229
• Page End: 2239
• Publication Date: 2020-01-20
• Subjects: c‐MYC -- G0 cell cycle re‐entry -- lung cancer -- structure‐specific recognition protein 1 -- suppressor of Ty homolog‐16; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.14897
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Blockade of cell cycle re‐entry in quiescent cancer cells is a strategy to prevent cancer progression and recurrence. We investigated the action and mode of action of CPF mixture ( C optis chinensis, P inellia ternata and F ructus trichosanthis ) in impeding a proliferative switch in quiescent lung cancer cells. The results indicated that CPF impeded cell cycle re‐entry in quiescent lung cancer cells by reduction of FACT and c‐MYC mRNA and protein levels, with concomitant decrease in H3K4 tri‐methylation and RNA polymerase II occupancy at FACT and c‐MYC promoter regions. Animals implanted with quiescent cancer cells that had been exposed to CPF had reduced tumour volume/weight. Thus, CPF suppresses proliferative switching through transcriptional suppression of FACT and the c‐MYC, providing a new insight into therapeutic target and intervention method in impeding cancer recurrence.