Electric cell-substrate impedance sensing in kidney research. Issue 2 (10th October 2019)

• Record Type: Journal Article
• Authors: Iwakura, Takamasa; Marschner, Julian A; Zhao, Zhi Bo; Świderska, Monika Katarzyna; Anders, Hans-Joachim
• Is Part Of: Nephrology dialysis transplantation. Volume 36:Issue 2(2021)
• Page Start: 216
• Page End: 223
• Publication Date: 2019-10-10
• Subjects: acute kidney injury -- cell culture -- chronic kidney disease -- stem cells -- technology; Nephrology -- Periodicals; Hemodialysis -- Periodicals; Kidneys -- Transplantation -- Periodicals; Hemodialysis; Kidneys -- Transplantation; Nephrology; Periodicals; 616.61
• DOI: 10.1093/ndt/gfz191
• Languages: English
• ISSNs: 0931-0509

Abstract

Abstract: Electric cell-substrate impedance sensing (ECIS) is a quantitative, label-free, non-invasive analytical method allowing continuous monitoring of the behaviour of adherent cells by online recording of transcellular impedance. ECIS offers a wide range of practical applications to study cell proliferation, migration, differentiation, toxicity and monolayer barrier integrity. All of these applications are relevant for basic kidney research, e.g. on endothelial cells, tubular and glomerular epithelial cells. This review gives an overview on the fundamental principles of the ECIS technology. We name strengths and remaining hurdles for practical applications, present an ECIS array reuse protocol, and review its past, present and potential future contributions to preclinical kidney research.