Development of an Analytical Method for Quantitation of Deoxynivalenol by UPLC–MS-MS: A Preliminary Assessment of Gestational and Lactational Transfer in Rats. Issue 6 (4th September 2020)

• Record Type: Journal Article
• Authors: Rehder Silinski, Melanie A; Gilliam, Jennifer A; Fernando, Reshan A; Robinson, Veronica G; Germolec, Dori; Cunny, Helen; Huang, Madelyn C; Furr, Johnathan; Waidyanatha, Suramya
• Is Part Of: Journal of analytical toxicology. Volume 45:Issue 6(2021)
• Page Start: 566
• Page End: 572
• Publication Date: 2020-09-04
• Subjects: Drugs -- Analysis -- Periodicals; Drugs -- Toxicity testing -- Periodicals; 615.907
• DOI: 10.1093/jat/bkaa119
• Languages: English
• ISSNs: 0146-4760

Abstract

Abstract: Deoxynivalenol (DON) is the most widely distributed trichothecene mycotoxin in grain-based foods and animal feed. Exposure to DON is widespread as it has been detected in food sources from around the world. The objective of this work was to develop a method to quantitate DON in biological matrices and apply it in a preliminary assessment of gestational and lactational transfer of DON following exposure of pregnant rats. The method used protein precipitation followed by ultra-performance liquid chromatography–tandem mass spectrometry. The method was evaluated in male Sprague Dawley rat plasma over the concentration range ∼2–1, 000 ng/mL. The method was linear (r ≥ 0.99), accurate (mean relative error ≤ ±4.9%) and precise (relative standard deviation ≤ 5.5%). The mean absolute recovery was 85.9%. The limit of detection was 0.35 ng/mL. The method was also evaluated in gestational day (GD) 18 Hsd:Sprague Dawley ® SD ® dam plasma and fetal homogenate (mean % relative error ≤ ±16.9; % relative standard deviation ≤ 9.5). Concentrations of DON in dam plasma stored at −80°C for at least 29 days and in fetal homogenate for at least 43 days were within 97.9 to 120% of Day 0 concentrations, demonstrating that DON is stable in these matrices. The method was used to quantitate DON in rat maternal plasma, amniotic fluid, GD 18 fetuses and postnatal day (PND) 4 pups following exposure of dams to 0 (control) and 1 mg/kg DON beginning on GD 6 and continuing through gestation and lactation for a preliminary assessment of maternal transfer. In animals exposed to 1 mg/kg/day, similar concentration of DON was found in GD 18 dam plasma and fetuses, demonstrating significant gestational transfer. The concentration of DON in PND 4 dam plasma was similar to that in GD 18 dam plasma. However, DON was not detected in PND 4 pup plasma above the limit of detection of the assay, demonstrating absence of transfer of DON to pups via lactation.