Sulfobacillus harzensis sp. nov., an acidophilic bacterium inhabiting mine tailings from a polymetallic mine. Issue 7 (8th July 2021)

• Record Type: Journal Article
• Authors: Zhang, Ruiyong; Hedrich, Sabrina; Jin, Decai; Breuker, Anja; Schippers, Axel
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 7(2021)
• Publication Date: 2021-07-08
• Subjects: acidophiles -- biomining -- iron- and sulfur-oxidation -- mine tailings -- Sulfobacillus; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004871
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A mixotrophic and acidophilic bacterial strain BGR 140 T was isolated from mine tailings in the Harz Mountains near Goslar, Germany. Cells of BGR 140 T were Gram-stain-positive, endospore-forming, motile and rod-shaped. BGR 140 T grew aerobically at 25–55 °C (optimum 45 °C) and at pH 1.5–5.0 (optimum pH 3.0). The results of analysis of the 16S rRNA gene sequences indicated that BGR 140 T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332 T, Sulfobacillus thermotolerans DSM 17362 T, and Sulfobacillus benefaciens DSM 19468 T were 94.8, 91.8 and 91.6 %, respectively. Its cell wall peptidoglycan is A1 γ, composed of meso -diaminopimelic acid. The respiratory quinone is DMK-6. The major polar lipids were determined to be glycolipid, phospholipid and phosphatidylglycerol. The predominant fatty acid is 11-cycloheptanoyl-undecanoate. The genomic DNA G+C content is 58.2 mol%. On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140 T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp. nov. is proposed because of its origin. Its type strain is BGR 140 T (=DSM 109850 T =JCM 39070 T ).