Polydopamine-enabled surface functionalization of gold nanorods for cancer cell-targeted imaging and photothermal therapy. (January 2013)

• Record Type: Journal Article
• Authors: Black, Kvar CL; Yi, Ji; Rivera, José G; Zelasko-Leon, Daria C; Messersmith, Phillip B
• Is Part Of: Nanomedicine. Volume 8:Number 1(2013)
• Page Start: 17
• Page End: 28
• Publication Date: 2013-01
• Subjects: antibody -- biomimetic adhesion -- EGF receptor -- mussel foot proteins -- nanoparticle -- optical imaging -- photothermal therapy -- plasmon -- surface modification; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.12.82
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: A novel biomimetic strategy was employed for presenting antibodies on gold nanorods (NRs) to target growth factor receptors on cancer cells for use in photothermal therapy.Materials & methods: Polydopamine (PD) was polymerized onto gold NRs, and EGF receptor antibodies (anti-EGFR) were immobilized onto the layer. Cell-binding affinity and light-activated cell death of cancer cells incubated with anti-EGFR-PD-NRs were quantified by optical imaging.Results: PD was deposited onto gold NRs, and antibodies were bound to PD-coated NRs. Anti-EGFR-PD-NRs were stable in media, and were specifically bound to EGFR-overexpressing cells. Illumination of cells targeted with anti-EGFR-PD-NRs enhanced cell death compared with nonirradiated controls and cells treated with antibody-free NRs.Conclusion: PD facilitates the surface functionalization of gold NRs with biomolecules, allowing cell targeting and photothermal killing of cancer cells. PD can potentially coat a large variety of nanoparticles with targeting ligands as a strategy for biofunctionalization of diagnostic and therapeutic nanoparticles. Original submitted 5 January 2012; Revised submitted 10 May 2012; Published online 14 August 2012