Sublethal effects of DBE-DBCH diastereomers on physiology, behavior, and gene expression of Daphnia magna. (1st September 2021)

• Record Type: Journal Article
• Authors: Seyoum, Asmerom; Kharlyngdoh, Joubert Banjop; Paylar, Berkay; Olsson, Per-Erik
• Is Part Of: Environmental pollution. Volume 284(2021)
• Publication Date: 2021-09-01
• Subjects: Brominated flame retardant -- Toxicogenomic -- Reproduction -- Longevity -- Gene expression -- TBECH; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2021.117091
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: 1, 2-dibromo-4-(1, 2-dibromoethyl)-cyclohexane (DBE-DBCH) is a brominated flame retardant used in commercial and industrial applications. The use of DBE-DBCH containing products has resulted in an increased release into the environment. However, limited information is available on the long-term effects of DBE-DBCH and its effects in aquatic invertebrates. Thus, the present study was aimed at determining how DBE-DBCH diastereomers (αβ and γδ) affects aquatic invertebrates using Daphnia magna as a model organism. Survival, reproduction, feeding, swimming behavior and toxicogenomic responses to environmental relevant concentrations of DBE-DBCH were analyzed. Chronic exposure to DBE-DBCH resulted in decreased lifespan, and reduced fecundity. Expression of genes involved in reproductive processes, vtg1 and jhe, were also inhibited. DBE-DBCH also induced hypoxia by inhibiting the transcription of genes involved in heme biosynthesis and oxygen transport. Furthermore, DBE-DBCH also inhibited feeding resulting in emptiness of the alimentary canal. Increased expression of the stress response biomarkers was observed following DBE-DBCH exposure. In addition, DBE-DBCH diastereomers also altered the swimming behavior of Daphnia magna . The present study demonstrates that DBE-DBCH cause multiple deleterious effects on Daphnia magna, including effects on reproduction and hormonal systems. These endocrine disrupting effects are in agreement with effects observed on vertebrates. Furthermore, as is the case in vertebrates, DBE-DBCH γδ exerted stronger effects than DBE-DBCH αβ on Daphnia magna. This indicate that DBE-DBCH γδ has properties making it more toxic to all so far studied animals than DBE-DBCH αβ. Graphical abstract: Image 1 Highlights: DBE-DBCH diastereomers affect Daphnia magna longevity, fecundity and behavior. Higher concentration of DBE-DBCH diastereomers inhibit feeding behavior of Daphnia magna. Daphnia magna lifespan is reduced by both DBE-DBCH αβ and DBE-DBCH γδ. DBE-DBCH diastereomers significantly alters swimming behavior of Daphnia magna. DBE-DBCH γδ has stronger effects on Daphnia magna than DBE-DBCH αβ.