Molecular cloning and characterization of the novel adropin from tilapia (Oreochromis niloticus): Involvement in the control of food intake. (August 2021)

• Record Type: Journal Article
• Authors: Zhang, Chaoyi; Yan, Yisha; Zhang, Qianli; Jiang, Quan
• Is Part Of: Neuropeptides. Volume 88(2021)
• Publication Date: 2021-08
• Subjects: Adropin -- Feeding control -- Gene regulation -- Tilapia; Neuropeptides -- Periodicals; Neuropeptides; Neuropeptides -- Périodiques; Neuropeptides; Electronic journals; Periodicals; 572.65
• DOI: 10.1016/j.npep.2021.102165
• Languages: English
• ISSNs: 0143-4179

Abstract

Abstract: Adropin has been shown to be involved in the regulation of food intake in mice. However, the mechanism of adropin in feeding regulation is still largely unknown. Using the tilapia, Oreochromis niloticus, we identified and characterized a novel form of adropin (designated adropin-b) encoding a 68-amino acid precursor. Although adropin-b shared low amino acid identities with its tilapia paralog (designated adropin-a), synteny analysis proved that tilapia adropin is orthologous to its human counterpart. The transcripts of adropin-b were ubiquitously expressed in various tissues with the highest levels in the olfactory bulb. A decrease in adropin-b mRNA levels was observed 1 h following a meal in the olfactory bulb, hypothalamus, and optic tectum, whereas fasting for 7 days induced an increase in adropin-b mRNA levels in the olfactory bulb, hypothalamus, and optic tectum of tilapia brain. However, no changes in adropin-a mRNA levels were observed in the postprandial and fasting state. Intraperitoneal injection of tilapia adropin-b was shown to increase food consumption, but adropin-a did not affect feeding. Co-treatment of the fish with adropin-b and neuropeptide Y (NPY) had no additive effects on appetite. The appetite stimulatory effects of adropin-b appeared to be mediated by upregulating the orexigenic Npy, Orexin, and Proapelin gene expression, paralleled by inhibition of the mRNA levels of anorexigenic proopiomelanocortin ( Pomc ) and cocaine-amphetamine-regulated transcript ( Cart ) in vivo and in vitro . These observations suggested that adropin-b participated in appetite control and gene regulation of central orexigenic and anorexigenic factors in a fish model. Graphical abstract: Unlabelled Image Highlights: A novel form of adropin (designated adropin-b) was identified in tilapia. The transcripts of adropin-b were abundantly expressed in brain areas. The postprandial and fasting state changed in adropin-b mRNA expression. Intraperitoneal injection of adropin-b stimulated food consumption in tilapia. Adropin-b could affect multiple orexigens and anorexigens.