Effect of prednisolone pre-treatment on cat lymphoma cell sensitivity towards chemotherapeutic drugs. (September 2021)

• Record Type: Journal Article
• Authors: Hlavaty, Juraj; Ertl, Reinhard; Mekuria, Tewodros Abere; Rütgen, Barbara; Tsujimoto, Hajime; Walter, Ingrid; Wolfesberger, Birgitt
• Is Part Of: Research in veterinary science. Volume 138(2021)
• Page Start: 178
• Page End: 187
• Publication Date: 2021-09
• Subjects: Corticosteroid treatment -- Drug resistance -- Feline lymphoma; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2021.06.014
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Corticosteroid administration prior to the application of chemotherapy in small animal lymphoma patients is a concern, as it is discussed to negatively influence the therapeutic outcome due to corticosteroid-induced drug resistance. Using feline lymphoma cell lines FT-1 and MS4 we have shown, that prednisolone pre-treatment alters the susceptibility of these cells towards doxorubicin or vincristine treatment in vitro . The observed effect was negative as for the killing potential and it was cell line and drug (doxorubicin or vincristine) dependent. Furthermore, increase in mRNA expression of selected proteins with multidrug resistance potential (MDR1, BCRP, LRP, MT) was observed after prednisolone pre-treatment. Administration of chemical inhibitors of these proteins did not lead to reversal in sensitivity of tested cell lines to doxorubicin or vincristine. Highlights: Corticosteroid-induced drug resistance in small companion animals is poorly elucidated with controversial clinical results published Prednisolone pre-treatment decreases the susceptibility of feline lymphoma cells towards doxorubicin or vincristine treatment in vitro Increased mRNA expression of proteins with multidrug resistance potential (MDR1, BCRP, LRP, MT) was observed after prednisolone pre-treatment Pre-treating lymphoma cats with prednisolone before starting chemotherapy should be considered carefully