Rice lectin protein r40c1 imparts drought tolerance by modulating S-adenosylmethionine synthase 2, stress-associated protein 8 and chromatin-associated proteins. (27th August 2020)

• Record Type: Journal Article
• Authors: Sahid, Salman; Roy, Chandan; Paul, Soumitra; Datta, Riddhi
• Editors: Gibbs, Daniel
• Is Part Of: Journal of experimental botany. Volume 71:Number 22(2020)
• Page Start: 7331
• Page End: 7346
• Publication Date: 2020-08-27
• Subjects: Drought -- lectin -- histone 4 -- OsMNB1B -- Osr40c1 -- S-adenosylmethionine synthase 2 -- stress-associated protein 8; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/eraa400
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract: Lectin proteins play an important role in biotic and abiotic stress responses in plants. Although the rice lectin protein Os r40c1 has been reported to be regulated by drought stress, the mechanism of its drought tolerance activity has not been studied so far. In this study, it is shown that expression of the Osr40c1 gene correlates with the drought tolerance potential of various rice cultivars. Transgenic rice plants overexpressing Osr40c1 were significantly more tolerant to drought stress than the wild-type plants. Furthermore, ectopic expression of the Osr40c1 gene in tobacco yielded a similar result. Interestingly, the protein displayed a nucleo-cytoplasmic localization and was found to interact with a number of drought-responsive proteins such as S -adenosylmethionine synthase 2 ( Os SAM2), stress-associated protein 8 ( Os SAP8), DNA-binding protein MNB1B ( Os MNB1B), and histone 4 ( Os H4). Silencing of each of these protein partners led to drought sensitivity in otherwise tolerant Os r40c1-expressing transgenic tobacco lines indicating that these partners were crucial for the Os r40c1-mediated drought tolerance in planta . Moreover, the association of Os r40c1 with these partners occurred specifically under drought stress forming a multi-protein complex. Together, our findings delineate a novel role of Os r40c1 in imparting drought tolerance by regulating Os MNB1B, Os SAM2, and Os H4 proteins, which presumably enables Os SAP8 to induce downstream gene expression. Abstract : A rice lectin protein, Os r40c1, plays a crucial role in imparting drought stress tolerance in plants by forming a multi-protein complex with Os SAM2, Os SAP8, Os MNB1B, Os H4, and two uncharacterized proteins.