Ratiometric Detection of Glutathione Based on Disulfide Linkage Rupture between a FRET Coumarin Donor and a Rhodamine Acceptor. (13th May 2021)

• Record Type: Journal Article
• Authors: Zhang, Yibin; Xia, Shuai; Wan, Shulin; Steenwinkel, Tessa E.; Vohs, Tara; Luck, Rudy L.; Werner, Thomas; Liu, Haiying
• Is Part Of: Chembiochem. Volume 22:Number 13(2021)
• Page Start: 2282
• Page End: 2291
• Publication Date: 2021-05-13
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202100108
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near‐infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide‐tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near‐infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self‐calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae. Abstract : A probe based on a coumarin donor and a near‐infrared rhodamine acceptor linked via a disulfide‐tethered bond has been developed for ratiometric visualization of glutathione in live cells and Drosophila melanogaster larvae through modulation of efficient Forster resonance energy transfer (FRET) from the donor to the acceptor.