Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis. Issue 3 (16th June 2021)

• Record Type: Journal Article
• Authors: Yassine, Edriss; Galiwango, Ronald; Ssengooba, Willy; Ashaba, Fred; Joloba, Moses L.; Zalwango, Sarah; Whalen, Christopher C.; Quinn, Frederick
• Is Part Of: MicrobiologyOpen. Volume 10:Issue 3(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-06-16
• Subjects: Mycobacterium tuberculosis -- single nucleotide polymorphism -- social network -- transmission -- tuberculosis; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.1211
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: Tuberculosis (TB) is the leading cause of death in humans by a single infectious agent worldwide with approximately two billion humans latently infected with the bacterium Mycobacterium tuberculosis . Currently, the accepted method for controlling the disease is Tuberculosis Directly Observed Treatment Shortcourse (TB‐DOTS). This program is not preventative and individuals may transmit disease before diagnosis, thus better understanding of disease transmission is essential. Using whole‐genome sequencing and single nucleotide polymorphism analysis, we analyzed genomes of 145 M . tuberculosis clinical isolates from active TB cases from the Rubaga Division of Kampala, Uganda. We established that these isolates grouped into M . tuberculosis complex (MTBC) lineages 1, 2, 3, and 4, with the most isolates grouping into lineage 4. Possible transmission pairs containing ≤12 SNPs were identified in lineages 1, 3, and 4 with the prevailing transmission in lineages 3 and 4. Furthermore, investigating DNA codon changes as a result of specific SNPs in prominent virulence genes including plcA and plcB could indicate potentially important modifications in protein function. Incorporating this analysis with corresponding epidemiological data may provide a blueprint for the integration of public health interventions to decrease TB transmission in a region. Abstract : By demonstrating that clear transmission relationships exist among groups of Mycobacterium tuberculosis clinical isolates via whole genome sequence comparisons and SNP threshold analysis, the corresponding epidemiological data can be used to confirm these linkages and ultimately provide an improved mechanism to design and implement control strategies within geographic regions such as Kampala, Uganda.