Establishment of quantitative methodology for sophoridine analysis and determination of its pharmacokinetics and bioavailability in rat. (4th May 2021)

• Record Type: Journal Article
• Authors: Chen, Mengchun; Jiang, Qi; Zhang, Mingyao; Chen, Sailing; Lou, Junsheng; Chen, Yijie; Wang, Fang; Wang, Rongyue
• Is Part Of: Drug development and industrial pharmacy. Volume 47:Number 5(2021)
• Page Start: 741
• Page End: 747
• Publication Date: 2021-05-04
• Subjects: Sophoridine -- UPLC–MS/MS -- rat plasma -- pharmacokinetics -- bioavailability; Pharmaceutical chemistry -- Periodicals; Pharmaceutical industry -- Periodicals; Drug Industry -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615.05
• DOI: 10.1080/03639045.2021.1934862
• Languages: English
• ISSNs: 0363-9045

Abstract

Abstract: Objective: The aim of this study is to develop a rapid and sensitive UPLC–MS/MS approach to determine the sophoridine (SOP) level in rat plasma and the pharmacokinetics of the substance. Significance: Sophoridine is used as an anti-inflammatory, anti-virus, anti-microbial, and anti-tumor alkaloid. It is essential to explore specific detection methods for the quantitative analysis of SOP in the blood circulation. Methods: The rat plasma samples were prepared by one-step protein precipitation with acetonitrile. Subsequently, the samples were separated by chromatography using a UPLC BEH C18 reversed-phase with an initial mobile phase of methanol and 0.1% formic acid aqueous solution. The gradient elution was performed at a fixed flow rate of 0.4 mL/min, and multiple reaction monitoring (MRM) mode with an electrospray positive ionization source was employed to detect the transitions of m/z 249.1 → 84.2 for SOP and m/z 264.3 → 69.8 for dendrobine (IS). The entire process required 3.5 min for each sample. Results: A linear correlation was established over the range of 2–2000 ng/mL ( r 2 ≥0.9954) for SOP in rat plasma with a lower limit of quantification (LLOQ) at 2 ng/mL. The range of accuracy was tested between 94.90% and 100.80%, and the relative standard deviations (RSDs) toward both intra- and inter-day precision were <10%. Thus, this method was successfully applied to a pharmacokinetic study, and the subsequent results demonstrated a low absolute bioavailability of 2.32%. Conclusion: The present study established a reliable method that quantified the SOP concentration in rat plasma after administering a dose of 2 mg/kg intravenously or 20 mg/kg orally.