A simple method to assay tumor cells based on target-initiated steric hindrance. Issue 53 (8th June 2021)

• Record Type: Journal Article
• Authors: Pan, Yanhong; Lu, Bing; Peng, Ying; Wang, Minghui; Deng, Ying; Yin, Yongmei; Yang, Jie; Li, Genxi
• Is Part Of: Chemical communications. Volume 57:Issue 53(2021)
• Page Start: 6522
• Page End: 6525
• Publication Date: 2021-06-08
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc02532e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We have proposed a simple electrochemical method in this work for the assay of tumor cells through their own steric hindrance effect. Abstract : We have proposed a simple electrochemical method in this work for the assay of tumor cells through their own steric hindrance effect. Specifically, tumor cells can block the catalysis of terminal deoxynucleotidyl transferase to the aptamer previously immobilized on the electrode surface. By making use of the hindrance effect, cancer cells can be quantitatively analyzed in the range from 1.6 × 10 2 to 1.6 × 10 6 cells per mL without complicated design or cumbersome operation, while the detection limit can be about 53 cells per mL. This method can also show satisfactory performance in complex environments, indicating its potential in clinical application.