Activation of anterior thalamic reticular nucleus GABAergic neurons promotes arousal from propofol anesthesia in mice. (30th April 2021)

• Record Type: Journal Article
• Authors: Liu, Yanjun; Chen, Bing; Cai, Yirong; Han, Yuan; Xia, Ying; Li, Nanqi; Fan, Bingqian; Yuan, Tianjie; Jiang, Junli; Gao, P o; Yu, Weifeng; Jiao, Yingfu; Li, Wenxian
• Is Part Of: Acta biochimica et biophysica Sinica. Volume 53:Number 7(2021)
• Page Start: 883
• Page End: 892
• Publication Date: 2021-04-30
• Subjects: thalamic reticular nucleus -- GABA -- propofol -- anesthesia; Biochemistry -- Periodicals; Biophysics -- Periodicals; 572.05
• DOI: 10.1093/abbs/gmab056
• Languages: English
• ISSNs: 1672-9145

Abstract

Abstract: Propofol is widely used for the induction and maintenance of anesthesia, which causes a rapid loss of consciousness. However, the mechanisms underlying the hypnosis effect of propofol are still not fully understood. The thalamic reticular nucleus (TRN) is crucial for regulating wakefulness, sleep rhythm generation, and sleep stability, while the role of TRN in the process of propofol-induced anesthesia is still unknown. Here, we investigated the function of the anterior TRN in propofol general anesthesia. Our results demonstrated that the neural activity of anterior TRN is suppressed during propofol anesthesia, whereas it is robustly activated from anesthesia by recording the calcium signals using fiber photometry technology. The results showed that the activation of anterior TRN neurons by chemogenetic and optogenetic methods shortens the emergency time without changing the induction time. Conversely, chemogenetic or optogenetic inhibition of the TRN neurons leads to a delay in the recovery time. Our study showed that anterior TRN is crucial for behavioral arousal without affecting the induction time of propofol anesthesia.