Feasibility of next-generation sequencing (Oncomine™ DX Target Test) for the screening of oncogenic mutations in advanced non-small-cell lung cancer patients. (21st April 2021)

• Record Type: Journal Article
• Authors: Takeyasu, Yuki; Yoshida, Tatsuya; Motoi, Noriko; Teishikata, Takashi; Tanaka, Midori; Matsumoto, Yuji; Shinno, Yuki; Okuma, Yusuke; Goto, Yasushi; Horinouchi, Hidehito; Kakishima, Hiroki; Tsuchida, Takaaki; Yamamoto, Noboru; Ohe, Yuichiro; Yatabe, Yasushi
• Is Part Of: Japanese journal of clinical oncology. Volume 51:Number 7(2021)
• Page Start: 1114
• Page End: 1122
• Publication Date: 2021-04-21
• Subjects: non-small-cell lung cancer -- next-generation sequencing -- Oncomine™ Dx Target Test -- targetable driver oncogene -- biomarker test; Oncology -- Periodicals; Cancer -- Periodicals; 616.994005
• DOI: 10.1093/jjco/hyab059
• Languages: English
• ISSNs: 0368-2811

Abstract

Abstract: Background: The Oncomine™ Dx Target Test based on next-generation sequencing has been approved for the screening of oncogenic mutations in advanced non-small-cell lung cancer patients. Methods: We assessed the tissue sample factors that affect the success rate of Oncomine™ Dx Target Test companion diagnostics and the feasibility of using biopsy specimens for Oncomine™ Dx Target Test companion diagnostics in advanced non-small-cell lung cancer patients. Results: Ninety-nine biopsy samples were subjected to genetic testing using the Oncomine™ Dx Target Test companion diagnostics to detect v-raf murine sarcoma viral oncogene homologue B1 mutations (Cohort 1), and 136 biopsy samples were examined using Oncomine™ Dx Target Test companion diagnostics for the detection of multiple oncogenic mutations (Cohort 2) between July 2018 and April 2020. We retrospectively collected clinical and pathological data, including tissue size and tumour cell content. The success rate was 77% (76/99) in Cohort 1 and 93% (127/136) in Cohort 2. In Cohort 1, the success rate was significantly associated with the tumour cell content: the success rate was 63% for samples with a tumour cell content of <20%, whereas it was 83% for samples with a tumour cell content of 20% or higher ( P = 0.0446). The tissue size also affected the success rate: a success rate of 57% was obtained for tissue sizes <4 mm 2, whereas a success rate of 95% was obtained for tissue sizes of 4 mm 2 or larger ( P < 0.0001). In Cohort 2, the success rate was 100% when tumour specimens with a tissue size of 4 mm 2 or larger were used. Conclusions: Tissue size and tumour cell content were significantly associated with the success rate of Oncomine™ Dx Target Test companion diagnostics. Abstract : We identified both tumour cell content (20% or more) and tissue sample size (4 mm 2 or more) affected the success rate of Oncomine™ Dx Target Test in patients with advanced non-small-cell lung cancer.