Determination of in vitro antioxidant activity and in vivo antineoplastic effects against Ehrlich ascites carcinoma of methanolic extract of Sphagneticola calendulacea (L.) Pruski. Issue 6 (June 2021)

• Record Type: Journal Article
• Authors: Bari, Md. Wasim; Islam, Ariful; Islam, Md. Monirul; Sultana, Mst Julia; Afroz, Rashida; Khan, Md. Mahbubur Rahman; Parul, Salina Shaheen; Swaraz, A.M.; Hossain, Mohammad Ismail; Islam, Mohammad Amirul
• Is Part Of: Heliyon. Volume 7:Issue 6(2021)
• Publication Date: 2021-06
• Subjects: Sphagneticola calendulacea -- Antioxidant activity -- Antineoplastic activity -- Ehrlich ascites carcinoma -- Apoptosis; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2021.e07228
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Background and aim: Sphagneticola calendulacea (L.) Pruski (S. calendulacea) is a well-known medicinal plant that is widely used in traditional medicine. This study was conducted to evaluate the antioxidant and antineoplastic properties of S. calendulacea leaf. Experimental procedure: Methanol was used as a solvent to prepare leaf extract from the dried leaf of S. calendulacea (MESL). We have evaluated the total phenolic and flavonoid contents of MESL, and using five different assays; we have also evaluated the antioxidant property of MESL. In vivo antineoplastic activity of MESL against Ehrlich ascites carcinoma (EAC) cells in experimental mice was also explored. Results: The phytochemical analysis of MESL exhibited the total phenolic, and total flavonoid contents in the dry extracts were 32.86 mg/gm of gallic acid equivalent and 127.26 mg/gm of catechin equivalent, respectively. Moreover, MESL also showed promising scavenging activity in all assays (IC50 value ≥70.5 μg/mL). In the in vivo antineoplastic assay, MESL showed increased EAC cell death at the doses of 50 mg/kg and 100 mg/kg body weight. MESL administration also induced apoptosis of EAC cells, significantly inhibited EAC cell growth, and increased the life span of EAC cell-bearing mice compared to EAC cell-bearing control mice. Conclusions: All findings of this study suggest potential antioxidant and antineoplastic properties of MESL. Abstract : Sphagneticola calendulacea; Antioxidant activity; Antineoplastic activity; Ehrlich ascites carcinoma; Apoptosis.