A case of fatal intoxication by ingestion of an herbicide formulation containing fluroxypyr-meptyl and triclopyr. (March 2021)

• Record Type: Journal Article
• Authors: Jang, Moonhee; Kim, Dongwoo; Yum, Hyesun; Moon, Sungmin; Kang, Minji; Kim, Byungjoo; Park, Jong-Pil; Choe, Sanggil
• Is Part Of: Forensic science international. Volume 320(2021)
• Publication Date: 2021-03
• Subjects: Fluroxypyr-meptyl -- Fluroxypyr -- Triclopyr -- Intoxication -- Blood -- Liquid chromatography–tandem mass spectrometry; Medical jurisprudence -- Periodicals; Chemistry, Forensic -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Chimie légale -- Périodiques; Gerechtelijke geneeskunde; Gerechtelijke chemie; Gerechtelijke psychiatrie; Chemistry, Forensic; Medical jurisprudence; Electronic journals; Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.forsciint.2021.110717
• Languages: English
• ISSNs: 0379-0738

Abstract

Abstract: Fluroxypyr-meptyl and triclopyr are synthetic auxin-like herbicides that are used to control woody and broadleaf weeds. Herein, we report a case of fatal intoxication involving fluroxypyr-meptyl and triclopyr. A 61-year-old man was found dead at his farm with several suicide notes, and a white plastic bottle and a plastic cup with traces of white emulsion were found next to him. The plastic bottle was labeled as an herbicide formulation containing fluroxypyr-meptyl and triclopyr. Forensic toxicological screening of the stomach contents revealed the presence of fluroxypyr-meptyl, fluroxypyr and triclopyr. However, no fluroxypyr-meptyl was detected in blood owing to its rapid hydrolysis to fluroxypyr. In this study, fluroxypyr and triclopyr in blood were extracted using solid-phase extraction, and analyzed by liquid chromatography–tandem mass spectrometry. The analytical method was validated in terms of linearity, precision, accuracy, recovery and matrix effect, and the acceptable criteria were satisfied. Toxicological analysis showed that fluroxypyr and triclopyr concentrations were 19.7 μg/mL and 137.4 μg/mL in peripheral blood and 16.5 μg/mL and 147.8 μg/mL in heart blood, respectively. Based on these toxicological results and autopsy findings, the cause of death was determined to be acute fatal intoxication by ingestion of the pesticide containing fluroxypyr-meptyl and triclopyr. This is the first report of the determination of fluroxypyr and triclopyr in a fatal intoxication case. Highlights: We report a fatal intoxication case of fluroxypyr and triclopyr. Fluroxypyr and triclopyr concentrations were determined in postmortem blood. The adjuvants might amplify the toxicity of fluroxypyr and triclopyr in this case.