Putative mechanism for anticancer properties of Ag–PP (NPs) extract. Issue 6 (24th June 2019)

• Record Type: Journal Article
• Authors: Homayouni‐Tabrizi, Masoud; Soltani, Mozhgan; Karimi, Ehsan; Namvar, Farideh; Pouresmaeil, Vahid; Es‐haghi, Ali
• Is Part Of: IET nanobiotechnology. Volume 13:Issue 6(2019)
• Page Start: 617
• Page End: 620
• Publication Date: 2019-06-24
• Subjects: nanomedicine -- genetics -- cellular biophysics -- toxicology -- patient treatment -- silver -- cancer -- biochemistry -- biomedical materials -- nanoparticles -- nanofabrication -- membranes; minimal side effects -- cancer treatment -- silver NPs -- cancer invasion -- vascular endothelial growth factor -- receptor genes -- VEGF‐R -- real‐time polymerase chain reaction assay -- angiogenesis effect -- chick chorioallantoic membrane assay -- MCF7 cells -- invasive cancer cells -- cytotoxic effects -- putative mechanism -- anticancer properties -- antiangiogenic effects -- antiangiogenesis properties -- Ag–PP‐induced cytotoxicity -- metastatic inhibition -- palm pollen extraction -- trypan blue testing -- time 48.0 hour -- Ag; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2018.5199
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : One of the most important challenges in treating cancer is the invasion and the angiogenesis of cancer cells. The synthesis of green nanoparticles (NPs) and their use in therapeutic fields is one of the most effective methods with minimal side effects in cancer treatment. In this study, cytotoxic and anti‐angiogenic effects of silver NPs (AgNPs) coated with palm pollen extract [Ag–PP(NPs)] were evaluated. For this purpose, the cells were treated with NPs and then were subjected to trypan blue testing (48 h). Then, the cancer invasion was evaluated by the scratch procedure and the expressions of the vascular endothelial growth factor (VEGF) and its receptor (VEGF‐R) genes were estimated using real‐time PCR assay. Also, the angiogenesis effect of the NPs was investigated with chick chorioallantoic membrane (CAM) assay. The Ag–PP(NPs) induced cytotoxicity on MCF7 cells. The findings also showed that Ag–PP(NPs) inhibit invasive cancer cells and reduce the expression of VEGF and VEGF‐R and significantly reduced the number and vessels lengths and the lengths and weights of the embryos in CAM assay. Ag–PP(NPs) with the induction of cytotoxic effects, metastatic inhibition and anti‐angiogenesis properties should be considered as an appropriate option for treatment of cancer