Nucleic acid detection strategy using gold nanoprobe of two diverse origin. Issue 9 (7th November 2019)

• Record Type: Journal Article
• Authors: Vinayagam, Saranya; Rajaiah, Paramasivan; Mukerjee, Amitava; Natarajan, Chandrasekaran
• Is Part Of: IET nanobiotechnology. Volume 13:Issue 9(2019)
• Page Start: 928
• Page End: 932
• Publication Date: 2019-11-07
• Subjects: patient diagnosis -- microorganisms -- nanomedicine -- gold -- fluorescence -- nanosensors -- DNA -- nanoparticles -- molecular biophysics -- diseases -- optical sensors; nucleic acid detection strategy -- gold nanoprobe -- silver nanoparticles -- lethal diseases -- dengue virus detection -- gold nanoparticles probe -- thiol tagged single strand DNA -- hybridisation process -- detection process -- aggregation -- complementary strand -- current detection method -- point‐of‐care diagnostic methods -- fluorescence‐based detection strategy -- Au; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2018.5332
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : In recent years, nanoparticles especially with gold and silver nanoparticles based point of care diagnostic methods is being developed for the lethal diseases like dengue. This study focused to work on the dengue virus detection in a simplest method using gold nanoparticles probe (AuNPs) with thiol tagged single strand DNA (ss‐DNA). A sensitive, fluorescence‐based detection strategy was designed to examine and quantified the hybridisation process and also elucidated the behaviour of AuNPs before and after interaction of biomolecule. The detection process was focused on aggregation of gold nanoprobe in the presence of complementary strand (target region). Hence the percentage of aggregation was measured and as a result, the limit of detection was found to be 10 −6 dilutions. Current detection method was highly sensitive, easy to perform and the reaction timing is rapid between 5 and 10 min, and it can be observed through naked eye.