Apoptotic efficacy and antiproliferative potential of silver nanoparticles synthesised from aqueous extract of sumac (Rhus coriaria L.). Issue 5 (27th March 2018)

• Record Type: Journal Article
• Authors: Ghorbani, Parisa; Namvar, Farideh; Homayouni‐Tabrizi, Masoud; Soltani, Mozhgan; Karimi, Ehsan; Yaghmaei, Parichehreh
• Is Part Of: IET nanobiotechnology. Volume 12:Issue 5(2018)
• Page Start: 600
• Page End: 603
• Publication Date: 2018-03-27
• Subjects: nanoparticles -- silver -- nanomedicine -- biomedical materials -- toxicology -- cancer -- molecular biophysics -- proteins -- biochemistry -- cellular biophysics -- nanofabrication; Ag -- Bcl‐2 down‐regulation -- Bax up‐regulation -- MCF‐7 cell viability -- concentration‐dependent inhibitory effect -- cytotoxic assay -- molecular analysis -- DAPI staining -- acridine orange‐propidium iodide staining -- morphological analysis -- MTT assay -- human breast cancer cells -- sumac fruit aqueous extract -- Anacardiaceae family -- cytotoxic effects -- drug delivery function -- diseases -- Rhus coriaria L -- silver nanoparticles -- antiproliferative potential -- apoptotic efficacy; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2017.0080
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Currently, nanotechnology and nanoparticles (NPs) are recognised due to their extensive applications in medicine and the treatment of certain diseases, including cancer. Silver NPs (AgNPs) synthesised by environmentally friendly method exhibit a high medical potential. This study was conducted to determine the cytotoxic and apoptotic effects of AgNPs synthesised from sumac (Anacardiaceae family) fruit aqueous extract (AgSu/NPs) on human breast cancer cells (MCF‐7). The anti‐proliferative effect of AgSu/NPs was determined by MTT assay. The apoptotic properties of AgSu/NPs were assessed by morphological analysis and acridine orange/propidium iodide (AO/PI) and DAPI staining. The mechanism of apoptosis induction in treated cells was investigated using molecular analysis. Overall results of morphological examination and cytotoxic assay revealed that AgSu/NPs exert a concentration‐dependent inhibitory effect on the viability of MCF‐7 cells (IC50 of ∼10 µmol/48 h). AO/PI staining confirmed the occurrence of apoptosis in cells treated with AgSu/NPs. In addition, molecular analysis demonstrated that the apoptosis in MCF‐7 cells exposed to AgSu/NPs was induced via up‐regulation of Bax and down‐regulation of Bcl‐2. These findings suggested the potential use of AgSu/NP as cytotoxic and pro‐apoptotic efficacy and its possible application in modern medicine for treating certain disorders, such as cancer.