Fe3 O4 /Ag nanocomposite biosynthesised using Spirulina platensis extract and its enhanced anticancer efficiency. Issue 7 (8th August 2019)

• Record Type: Journal Article
• Authors: Salehzadeh, Ali; Naeemi, Akram Sadat; Khaknezhad, Ladan; Moradi‐Shoeili, Zeinab; Shandiz, Seyed Ataollah Sadat
• Is Part Of: IET nanobiotechnology. Volume 13:Issue 7(2019)
• Page Start: 766
• Page End: 770
• Publication Date: 2019-08-08
• Subjects: nanomedicine -- nanocomposites -- toxicology -- cancer -- drug delivery systems -- nanofabrication -- cellular biophysics -- nanoparticles; MCF‐7 cells -- 5‐diphenyl‐tetrazolium -- apoptotic effects -- propidium iodide staining -- dose‐dependent cell proliferation reduction -- apoptotic cells -- untreated cells -- nanocomposite -- Hoechst 33258 staining method -- human breast cancer cells -- physico‐chemical properties -- spectroscopic methods -- in vitro cytotoxic effect -- in vitro anticancer activity -- biosynthesis -- caspase 3 activation assays -- annexin V‐fluorescein isothiocyanate -- FITC -- Fe3 O4 ‐Ag; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2018.5364
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : In this work, the authors investigated the apoptotic activities of Fe3 O4 /Ag nanocomposite biosynthesised by Spirulina platensis extract against MCF‐7 (human breast cancer cells). The physico‐chemical properties of prepared Fe3 O4 /Ag nanocomposite were studied by different spectroscopic methods. To evaluate the in vitro cytotoxic effect, MCF‐7 cells were treated with different concentrations of Fe3 O4 /Ag nanocomposite and examined by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl‐tetrazolium bromide (MTT) assay. Moreover, apoptotic effects were also studied by Hoechst 33258 staining, caspase 3 activation assays, and annexin V‐fluorescein isothiocyanate (FITC) and propidium iodide staining. Microscopic observations of Fe3 O4 /Ag nanocomposites indicated approximately spherical shape and small particles in the size range of about 30–50 nm. The MTT assay result revealed that the Fe3 O4 /Ag nanocomposite causes a dose‐dependent cell proliferation reduction in MCF‐7 cells (IC50 = 135 μg/ml). Regarding to the Annexin V/PI staining result, the increase percentage of apoptotic cells (28.09%) was detected as compared to untreated cells. According to the caspase assay, Fe3 O4 /Ag nanocomposite enhances caspase 3 level. Furthermore, in vitro anti‐cancer activity of the nanocomposite was performed by Hoechst 33258 staining method. The proposed data suggest that Fe3 O4 /Ag nanocomposite may be an effective agent for the inhibition of breast cancer cells at in vitro level.