Amino‐functionalised mesoporous silica microspheres for immobilisation of Candida antarctica lipase B – application towards greener production of 2, 5‐furandicarboxylic acid. Issue 8 (29th September 2020)

• Record Type: Journal Article
• Authors: Saikia, Kongkona; Senthil Kumar, Ponnusamy; Karanam Rathankumar, Abiram; SaiLavanyaa, Sundar; Srinivasan, Lakshmi; Subramanian, Sivanesan; Cabana, Hubert; Gosselin, Mathilde; Vinoth Kumar, Vaidyanathan
• Is Part Of: IET nanobiotechnology. Volume 14:Issue 8(2020)
• Page Start: 732
• Page End: 738
• Publication Date: 2020-09-29
• Subjects: mesoporous materials -- organic compounds -- biotechnology -- silicon compounds -- renewable materials -- catalysis -- catalysts -- enzymes -- thermal stability -- biofuel; FDCA production -- amino‐functionalised mesoporous silica microspheres -- greener production -- 2, 5‐furandicarboxylic acid -- covalent immobilisation -- 2, 5‐diformylfuran -- CaLB immobilisation -- Candida antarctica lipase B immobilisation -- ethyl butyrate -- time 48.0 hour -- temperature 30.0 degC -- time 12.25 hour; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2020.0021
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : In the present study, amino‐functionalised mesoporous silica microspheres were utilised as support for the covalent immobilisation of Candida antarctica lipase B (CaLB) for the subsequent production of 2, 5‐furandicarboxylic acid (FDCA) from 2, 5‐diformylfuran (DFF). Under the optimised operating conditions of pH 6.5, particle/enzyme ratio of 1.25:1.0 and glutaraldehyde concentration of 4 mM, a maximum CaLB immobilisation yield of 82.4% on silica microspheres was obtained in 12.25 h. The immobilised CaLB was used for the synthesis of alkyl esters, which were utilised along with hydrogen peroxide for FDCA synthesis. The biocatalytic conversion of 30 mM DFF dictated a 77–79% FDCA in 48 h at 30°C; where the turnover number and turnover frequency of immobilised CaLB were 6220.73 mol mol −1 and 129.59 h −1, respectively, for ethyl acetate, against 6297.65 mol mol −1 and 131.2 h −1, respectively, for ethyl butyrate. Upon examining the operational stability, the immobilised CaLB exhibited high stability till five cycles of FDCA production.