DOC‐LS, a new liposome for dermal delivery, and its endocytosis by HaCaT and CCC‐ESF‐1 cells. Issue 8 (24th October 2018)

• Record Type: Journal Article
• Authors: Zhang, Yong‐Tai; Zhang, Kai; Li, Zhe; Zhang, Hong‐Yu; Guo, Teng; Li, Yan‐Yan; Zhao, Ji‐Hui; Feng, Nian‐Ping
• Is Part Of: IET nanobiotechnology. Volume 12:Issue 8(2018)
• Page Start: 1037
• Page End: 1041
• Publication Date: 2018-10-24
• Subjects: drugs -- nanoparticles -- lipid bilayers -- nanomedicine -- biomedical materials -- electrokinetic effects -- biomembrane transport -- drug delivery systems -- skin -- organic compounds; dermal delivery -- CCC‐ESF‐1 cells -- skin cells -- deoxycholate‐mediated liposomes -- coumarin 6‐loaded DOC‐LS -- endocytosis inhibitors -- clathrin‐mediated endocytic pathways -- endocytosis pathways -- HaCaT cell lines -- size distribution -- zeta potential -- nanocarriers -- flow cytometry -- pinocytotic vesicles -- macropinocytosis -- microneedle system -- efficient intracellular drug delivery -- targeting cutaneous histocyte disorders; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2018.5079
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : The main objective of this work was to investigate the uptake channels of skin cells through which coumarin 6, transported by deoxycholate‐mediated liposomes (DOC‐LS), was internalised; this was also compared against the action of conventional LS. Coumarin 6‐loaded DOC‐LS and LS were characterised for size distribution, zeta potential, and shape, and analysed in vitro in human epidermal immortal keratinocyte (HaCaT) (epidermal) and human embryonic skin fibroblast (CCC‐ESF‐1) (dermal) cell lines. Various endocytosis inhibitors were incubated with cells treated with the nanocarriers. Flow cytometry results indicated that HaCaT and CCC‐ESF‐1 cells internalise the tested preparations through pinocytotic vesicles, macropinocytosis, clathrin‐mediated endocytic pathways, and via lysosomes, which consume a considerable amount of energy. The endocytosis pathways of DOC‐LS and LS showed no difference. This study provides a basis for the application of LS being combined with a microneedle system for efficient intracellular drug delivery, targeting cutaneous histocyte disorders.