Method development for optimised green synthesis of gold nanoparticles from Millettia pinnata and their activity in non‐small cell lung cancer cell lines. Issue 6 (4th July 2019)

• Record Type: Journal Article
• Authors: Kumar, Gourav; Ghosh, Manik; Pandey, Dev Mani
• Is Part Of: IET nanobiotechnology. Volume 13:Issue 6(2019)
• Page Start: 626
• Page End: 633
• Publication Date: 2019-07-04
• Subjects: nanomedicine -- molecular biophysics -- cancer -- electrokinetic effects -- pH -- transmission electron microscopy -- toxicology -- gold -- cellular biophysics -- X‐ray diffraction -- lung -- biomedical materials -- nanofabrication -- nanoparticles -- particle size -- enzymes -- visible spectra -- ultraviolet spectra -- light scattering -- biochemistry; method development -- optimised green synthesis -- gold nanoparticles -- millettia pinnata -- nonsmall cell lung cancer cell lines -- plant extracts -- single step reaction -- shade‐dried leaf extracts -- aqueous auric chloride -- synthesis method -- stable GNPs -- monodispersed GNPs -- spherical‐shaped nanoparticles -- A549 lung cancer cells -- green source -- particle size -- enzyme concentration -- substrate concentration -- biomolecules -- reaction time -- UV‐visible‐absorption spectroscopy -- dynamic light scattering -- zeta potential -- TEM images -- powder XRD -- face‐centred cubic arrangement -- cytotoxicity -- pH -- therapeutic agents -- drug delivery carriers -- time 12.0 hour -- Au; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2018.5410
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Green synthesis of gold nanoparticles (GNPs) has received substantial attention, because nanoparticles are produced in an eco‐friendly way using biomolecules present in plant extracts in a single step reaction. This research article highlights GNPs obtained using shade‐dried leaf extracts of Millettia pinnata (L.) with aqueous auric chloride (HAuCl4 ) at ambient temperature. In the present study, GNPs with average particle size 37 nm in size were fabricated. Furthermore, the synthesis method to obtain stable and monodispersed GNPs was advanced by optimising enzyme concentration 100 μg/ml, pH 5.4, substrate concentration 0.45 mM and 12 h time of reaction. The confirmation of GNPs formation and characterisation was followed by UV‐vis‐absorption spectroscopy, dynamic light scattering (DLS), and zeta potential (ZP) for the analysis of shape, size, and stability, respectively. TEM images and powder XRD revealed the GNPs synthesis of spherical‐shaped nanoparticles in the face‐centred cubic arrangement. Cytotoxicity of GNPs was studied against A549 lung cancer cells with IC50 14.76 μg/ml and found lower as compared to doxorubicin IC50 11.23 μg/ml but significant enough to be used as a vehicle GNPs produced using green source can be used as significant therapeutic agents and drug delivery carriers.