Green synthesis of silver nanoparticles using Zingiber officinale and Thymus vulgaris extracts: characterisation, cell cytotoxicity, and its antifungal activity against Candida albicans in comparison to fluconazole. Issue 2 (9th January 2019)

• Record Type: Journal Article
• Authors: Mohammadi, Mohsen; Shahisaraee, Sabrieh Assadi; Tavajjohi, Atiyeh; Pournoori, Negin; Muhammadnejad, Samad; Mohammadi, Shahla Roodbar; Poursalehi, Reza; Delavari H, Hamid
• Is Part Of: IET nanobiotechnology. Volume 13:Issue 2(2019)
• Page Start: 114
• Page End: 119
• Publication Date: 2019-01-09
• Subjects: nanoparticles -- surface plasmon resonance -- adsorption -- nanofabrication -- particle size -- silver -- ultraviolet spectra -- antibacterial activity -- visible spectra -- microorganisms -- nanomedicine -- Fourier transform infrared spectra -- biomedical materials -- diseases -- materials preparation -- cellular biophysics; green synthesis -- cell cytotoxicity -- antifungal activity -- fluconazole application -- FLZ‐resistant candida -- green synthesised silver nanoparticles -- antifungal agent -- surface coating -- surface plasmon resonance -- superficial fungal infections -- Zingiber officinale -- UV‐visible spectroscopy -- Thymus vulgaris extracts -- antifungal azole agent -- Candida albicans fungal pathogen -- plant extracts -- ginger -- Fourier transform infrared spectroscopy -- minimum inhibitory concentration method -- Ag; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2018.5146
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : Fluconazole (FLZ) application as a highly successful commercial antifungal azole agent to treat the fungal infections is limited due to emergence of FLZ‐resistant candida. In this study, the potential of green synthesised silver nanoparticles (NPs) as an antifungal agent against Candida albicans fungal pathogen is investigated. The extract of ginger ( Zingiber officinale ) and thyme ( Thymus vulgaris ) plays as reducing agent, capping agent and antifungal agent. The UV–visible spectroscopy shows the peak of surface plasmon resonance of synthesised Ag NPs after a period of time. The synthesised Ag NPs are spherical, with average sizes of 12 and 18 nm based on ginger and thyme extract, respectively. Fourier transform infrared spectroscopy confirms the adsorption of the plant extract on the surface of the as‐prepared Ag NPs. Based on the minimum inhibitory concentration (MIC) method against Candida albicans, the antifungal activity of as‐prepared green synthesised Ag NPs shows higher inhibitory in comparison to FLZ. Finally, the Ag NPs synthesised via thyme extract shows no cytotoxicity with concentration below 3.5 ppm, which can be considered as an appropriate candidate instead of FLZ to treat the superficial fungal infections.