Formulation and characterisation of a self‐nanoemulsifying drug delivery system of amphotericin B for the treatment of leishmaniasis. Issue 5 (17th April 2019)

• Record Type: Journal Article
• Authors: Khan, Momin; Nadhman, Akhtar; Shah, Walayat; Khan, Imran; Yasinzai, Masoom
• Is Part Of: IET nanobiotechnology. Volume 13:Issue 5(2019)
• Page Start: 477
• Page End: 483
• Publication Date: 2019-04-17
• Subjects: nanomedicine -- drops -- microorganisms -- electrokinetic effects -- cellular biophysics -- drug delivery systems -- monolayers -- drugs -- diseases; self‐nanoemulsifying drug delivery system -- topical routes -- oral routes -- SNEDDS formulation -- mucus permeation study -- cell permeation -- leishmaniasis treatment -- amphotericin B -- zeta potential -- Caco‐2 cell monolayer -- vortex -- sonication -- droplet size -- Caco‐2 cell viability -- antileishmanial activity -- promastigotes -- amastigotes -- Leishmania parasites; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2018.5281
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : This study was aimed to develop a self‐nanoemulsifying drug delivery system (SNEDDS) for amphotericin B (AmB) potential use in leishmaniasis through topical and oral routes. Two formulations, formulation A and formulation B (FA and FB) of AmB loaded SNEDDS were developed by mixing their excipients through vortex and sonication. The SNEDDS formulation FA and FB displayed a mean droplet size of 27.70 ± 0.5 and 30.17 ± 0.7 nm and zeta potential −11.4 ± 3.25 and −13.6 ± 2.75 mV, respectively. The mucus permeation study showed that formulation FA and FB diffused 1.45 and 1.37%, respectively in up to 8 mm of mucus. The cell permeation across Caco‐2 cells monolayer was 10 and 11%, respectively. Viability of Caco‐2 cells was 89% for FA and 86.9% for FB. The anti‐leishmanial activities of FA in terms of IC50 were 0.017 µg/ml against promastigotes and 0.025 µg/ml against amastigotes, while IC50 values of FB were 0.031 and 0.056 µg/ml, respectively. FA and FB killed macrophage harboured Leishmania parasites in a dose‐dependent manner and a concentration of 0.1 µg/ml killed 100% of the parasites. These formulations have the potential to provide a promising tool for AmB use through oral and topical routes in leishmaniasis therapy.