A simple downstream processing protocol for the recovery of lactic acid from the fermentation broth. (December 2020)

• Record Type: Journal Article
• Authors: Kumar, Sumit; Yadav, Neerja; Nain, Lata; Khare, Sunil Kumar
• Is Part Of: Bioresource technology. Volume 318(2020)
• Publication Date: 2020-12
• Subjects: Lactic acid -- Downstream process -- Lactic acid bacteria (LAB) -- Solvent extraction -- Fermentation broth; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124260
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: A simple and economical process for downstream of lactic acid has been developed. The pH of the extraction medium was critical during lactic acid purification. Optimized downstream process conditions resulted in a yield of 86% and 93% purity. The purified lactic acid was further characterized by FTIR and NMR. Abstract: Lactic acid is one of the essential platform chemicals, and despite the availability of a range of downstream processes, its effective recovery is still elusive. A phase partitioning process using n-butanol and a chaotropic salt ammonium sulphate was developed to recover lactic acid from the fermentation broth of Lactobacillus pentosus SKL-18. During the optimization of various process parameters, the extraction medium's pH was found to be critical, with 2.5 being the best. The optimized process resulted in a lactic acid yield of 86% and found it to be 93% pure. The purity and characteristics of lactic acid were confirmed by FTIR and NMR spectra. This solvent-based extraction procedure is an economical and straightforward downstream process for purifying lactic acid produced from agro- and bakery-residues. The pure lactic acid can further be used for enzymatic synthesis of high value-added product PLA, a biodegradable and biocompatible plastics.