Antimicrobial-resistance profiles of gram-negative bacteria isolated from green turtles (Chelonia mydas) in Taiwan. (15th May 2021)

• Record Type: Journal Article
• Authors: Tsai, Ming-An; Chang, Chao-Chin; Li, Tsung-Hsien
• Is Part Of: Environmental pollution. Volume 277(2021)
• Publication Date: 2021-05-15
• Subjects: Antimicrobials -- Chelonia mydas -- Antimicrobial-resistant bacteria; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2021.116870
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The green turtle ( Chelonia mydas ) is listed as a globally endangered species and is vulnerable to anthropogenic threats, including environmental pollution. This study investigated the antimicrobial resistance of Gram-negative bacteria isolated from wild green turtles admitted to a sea turtle rehabilitation center in Taiwan. For this investigation, cloacal and nasal swab samples were collected from 28 green turtles between 2018 and 2020, from which a total of 47 Gram-negative bacterial isolates were identified. Among these, Vibrio spp. were the most dominant isolate (31.91%), and 89.36% of the 47 isolates showed resistance to at least one of 18 antimicrobial agents tested. Isolates resistant to one (6.38%), two (8.51%), and multiple (74.47%) antimicrobials were observed. The antimicrobial agents to which isolates showed the greatest resistance were penicillin (74.47%), followed by spiramycin, amoxicillin, and cephalexin. The antimicrobial-resistance profiles identified in this study provide useful information for the clinical treatment of sea turtles in rehabilitation facilities. The results of our study also imply that wild green turtles may be exposed to polluting effluents containing antimicrobials when the turtles traverse migratory corridors or forage in feeding habitats. To benefit sea turtle conservation, future research should focus on (1) how to prevent pollution from antimicrobials in major green turtle activity areas and (2) identifying sources of antimicrobial-resistant bacterial strains in coastal waters of Taiwan. Graphical abstract: High proportion of Gram-negative bacteria from green turtles with multiple (i.e., resistance to at least three antimicrobials) antimicrobial resistance. Image 1 Highlights: This is the first study to show antimicrobial resistance in Gram-negative bacteria from green turtles in East Asia. 74.4% of Gram-negative bacteria from green turtles were resistant to at least three antimicrobial agents. Antimicrobial-resistant bacteria in sea turtles raises concerns about the extent of antimicrobial pollution in waters. Abstract : The multiple antimicrobial-resistant bacteria identified in endangered green turtles in Taiwan suggest that this marine species may be potentially exposed to marine pollutions including antimicrobials.