Ammonium detoxification mechanism of ammonium-tolerant duckweed (Landoltia punctata) revealed by carbon and nitrogen metabolism under ammonium stress. (15th May 2021)

• Record Type: Journal Article
• Authors: Tian, Xueping; Fang, Yang; Jin, Yanling; Yi, Zhuolin; Li, Jinmeng; Du, Anping; He, Kaize; Huang, Yuhong; Zhao, Hai
• Is Part Of: Environmental pollution. Volume 277(2021)
• Publication Date: 2021-05-15
• Subjects: Ion transport -- Ammonium assimilation -- Starch -- Carbon skeletons -- Transcriptome sequencing; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2021.116834
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: In this work, the ammonium-tolerant duckweed Landoltia punctata 0202 was used to study the effect of ammonium stress on carbon and nitrogen metabolism and elucidate the detoxification mechanism. The growth status, protein and starch content, and activity of nitrogen assimilation enzymes were determined, and the transcriptional levels of genes involved in ion transport and carbon and nitrogen metabolism were investigated. Under high ammonium stress, the duckweed growth was inhibited, especially when ammonium was the sole nitrogen source. Ammonium might mainly enter cells via low-affinity transporters. The stimulation of potassium transport genes suggested sufficient potassium acquisition, precluding cation deficiency. In addition, the up-regulation of ammonium assimilation and transamination indicated that excess ammonium could be incorporated into organic nitrogen. Furthermore, the starch content increased from 3.97% to 16.43% and 26.02% in the mixed-nitrogen and ammonium-nitrogen groups, respectively. And the up-regulated starch synthesis, degradation, and glycolysis processes indicated that the accumulated starch could provide sufficient carbon skeletons for excess ammonium assimilation. The findings of this study illustrated that the coordination of carbon and nitrogen metabolism played a vital role in the ammonium detoxification mechanism of duckweeds. Graphical abstract: Image 1 Highlights: NH4 + inhibited growth but promoted starch accumulation of duckweed. NH4 + enters cells via low-affinity transporters under high ammonium stress. The stimulation of K + transporters could ensure sufficient cations acquisition. High NH4 + concentration stimulated assimilation and transamination significantly. Starch degradation could provide carbon skeletons for excess NH4 + assimilation. Abstract : Sufficient cation acquisition, enhanced nitrogen assimilation and starch synthesis and catabolism played a vital role in the NH4 + detoxification of duckweeds.