ERβ regulated ovarian kisspeptin plays an important role in oocyte maturation. (1st May 2021)

• Record Type: Journal Article
• Authors: Chakravarthi, V. Praveen; Ghosh, Subhra; Housami, Sami M.; Wang, Huizhen; Roby, Katherine F.; Wolfe, Michael W.; Kinsey, William H.; Rumi, M.A. Karim
• Is Part Of: Molecular and cellular endocrinology. Volume 527(2021)
• Publication Date: 2021-05-01
• Subjects: Kisspeptin -- Kisspeptin receptor -- Estrogen receptor β -- Gonadotropin -- Granulosa cells -- And oocyte maturation; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2021.111208
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Kisspeptin (KISS1) signaling in the hypothalamic-pituitary (H–P) axis plays an essential role in regulating gonadotropin secretion. KISS1 and KISS1 receptor (KISS1R) are also expressed in the ovary; however, the role of intraovarian KISS1 signaling remains unclear. Granulosa cell (GC)-specific expression of KISS1, and oocyte-specific expression of KISS1R indicate that GC-derived KISS1 may act on oocytes. Expression of KISS1 in GCs is induced by gonadotropins but it is absent in estrogen receptor β knockout ( Erβ null ) rat ovaries. We also observed that gonadotropin stimulation failed to induce maturation of Erβ null oocytes. Interestingly, KISS1 treatment of cumulus oocyte complexes (COCs) isolated from antral follicles promotes in vitro maturation of oocytes. Treatment of oocytes with KISS1 induced intracellular Ca 2+ release, and increased activation of MAP kinase ERK1/2. KISS1 treatment also induced the expression of oocyte genes that are crucial for differentiation of GCs, and maturation of oocytes. Our findings suggest that ovarian KISS1-signaling plays an important role in gonadotropin induced follicle development and oocyte maturation. Highlights: Expression of Kiss1 is predominant in rat granulosa cells whereas the expression of Kiss1r is remarkably high in oocytes. Granulosa cell derived kisspeptins may act on oocytes in regulating the maturation. Kisspeptin signaling induces intracellular Ca 2+ release followed by activation of MAP kinase pathway in oocytes. Kisspeptin signaling also induces the expression of key genes involved in oocyte maturation.