Production and high throughput quantification of fucoxanthin and lipids in Tisochrysis lutea using single-cell fluorescence. (December 2020)

• Record Type: Journal Article
• Authors: Gao, Fengzheng; Teles (Cabanelas, ITD), Iago; Ferrer-Ledo, Narcís; Wijffels, René H.; Barbosa, Maria J.
• Is Part Of: Bioresource technology. Volume 318(2020)
• Publication Date: 2020-12
• Subjects: Fucoxanthin -- Single-cell -- Fluorescence-activated cell sorting (FACS) -- Polar/neutral lipids -- Chlorophyll; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124104
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Fucoxanthin is positively correlated with chlorophyll a and polar lipids. The polarity in the lipid content can be monitored using FACS. FACS can be used to quantify pigments and lipids and sort single T. lutea cells. Abstract: This work aimed to investigate the accumulation of fucoxanthin and lipids in Tisochrysis lutea during growth (N+) and nitrogen-starvation (N−) and to correlate these products with single-cell emissions using fluorescence-activated cell sorting (FACS). Fucoxanthin content decreased 52.94% from N+ to N− in batch cultivation; increased 40.53% as dilution rate changed from 0.16 to 0.55 d −1 in continuous cultivation. Total lipids (N−) were constant (~250 mg/g), but the abundance of neutral lipids increased from 4.87% to 40.63%. Nile red can stain both polar and neutral lipids. However, in vivo, this differentiation is limited due to an overlapping of signals between 600 and 660 nm, caused by neutral lipids concentrations above 3.48% (W/W). Chlorophyll autofluorescence (720 nm) was reported for the first time as a proxy for fucoxanthin (R 2 = 0.90) and polar lipids (R 2 = 0.98). FACS can be used in high throughput quantification of pigments and lipids and to select and sort cells with high-fucoxanthin/lipids.