A polyphenol-rich prebiotic in combination with a novel probiotic formulation alleviates markers of obesity and diabetes in Drosophila. (September 2018)

• Record Type: Journal Article
• Authors: Westfall, Susan; Lomis, Nikita; Prakash, Satya
• Is Part Of: Journal of functional foods. Volume 48(2018)
• Page Start: 374
• Page End: 386
• Publication Date: 2018-09
• Subjects: Probiotic -- Synbiotic -- Gut microbiota -- Diabetes -- Obesity -- TOR; ACC acetyl CoA-carboxylase -- Akt Protein kinase B -- AMPK 5′-adenosine monophosphate-activated protein kinase -- Bi702255 Bifidobacteria longum spp. infantis NCIMB 702255 -- BMI Body mass index -- CFU colony forming units -- Dilp Drosophila insulin-like peptide -- FA ferulic acid -- FAS fatty acid synthase -- FFAR free fatty acid receptor -- FIAF fasting-induced adipose factor -- FOS fructooligosaccharide -- FOXO Forkhead Box-O -- GIT gastrointestinal tract -- GLP-1 glucagon-like protein 1 -- GLUT4 glucose transporter 4 -- GOS galactooligosaccharide -- GPR G-protein receptor -- HFD high fat diet -- IBS irritable bowel syndrome -- InR insulin receptor -- Lf5221 Lactobacillus fermentum NCIMB 5221 -- Lp8826 Lactobacillus plantarum NCIMB 8826 -- MRS Man-Rogosa-Sharpe -- NCIMB National Collection of Industrial Food and Marine Bacteria -- PEPCK phosphoenol pyruvate carboxykinase -- PPAR peroxisome proliferator-activated receptor -- ROS reactive oxygen species -- SCFAs short-chain fatty acids -- SREBP sterol regulatory element-binding protein -- T2D type 2 diabetes -- TFLA triphala -- TOR target of rapamycin -- TZDs thiazolidinediones -- XOS xylooligosaccharides; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.07.012
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: A synbiotic formulation alleviates multiple markers of diet-induced diabetes and obesity. The probiotic and synbiotic formulations have combinatorial metabolic action. dTOR and PPARy are potential regulatory targets of the synbiotic formulation. Synbiotics are potential therapeutic agents for diabetes and obesity. Abstract: Imbalances in the gut microbiota are directly associated with the pathogenesis of diabetes and obesity. The current study investigates how the combination of three probiotics Lactobacillus plantarum NCIMB 8826, L. fermentum NCIMB 5221 and Bifidobacteria longum subsp. infantis NCIMB 702255 with a novel polyphenol-rich prebiotic Triphala has combinatorial benefits on the symptoms and underlying mechanisms of diet-induced diabetes and obesity. Drosophila melanogaster fed either a high-fat (HFD) or high-sugar diet (HSD) were pretreated with individual or combined probiotic and/or prebiotic therapy. The individual probiotics elicited some beneficial effects on the physiological markers of diabetes and obesity including reductions in total weight, glucose and triglyceride levels with Lp8826 having a higher efficacy against HSD and Bi702255 against HFD stresses; however, a combinatorial effect was observed with the probiotic and synbiotic formulations. Further, the synbiotic formulation consistently rescued variations in insulin resistance and lipogenesis outlining the synbiotic's potential for treating metabolic disorders.