Abscisic Acid Modulates Seed Germination via ABA INSENSITIVE5-Mediated PHOSPHATE1. Issue 4 (31st October 2017)

• Record Type: Journal Article
• Authors: Huang, Yun; Sun, Mi-Mi; Ye, Qing; Wu, Xiao-Qing; Wu, Wei-Hua; Chen, Yi-Fang
• Is Part Of: Plant physiology. Volume 175:Issue 4(2017)
• Page Start: 1661
• Page End: 1668
• Publication Date: 2017-10-31
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.17.00164
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Arabidopsis PHO1 modulates ABA-mediated seed germination and is a key target of transcription factor ABI5. Abstract: The phytohormone abscisic acid (ABA) controls many developmental and physiological processes. Here, we report that PHOSPHATE1 (PHO1) participates in ABA-mediated seed germination and early seedling development. The transcription of PHO1 was obviously enhanced during seed germination and early seedling development and repressed by exogenous ABA. The pho1 mutants ( pho1-2, pho1-4, and pho1-5 ) showed ABA-hypersensitive phenotypes, whereas the PHO1 -overexpressing lines were ABA-insensitive during seed germination and early seedling development. The expression of PHO1 was repressed in the ABI5 -overexpressing line and elevated in the abi5 mutant, and ABI5 can bind to the PHO1 promoter in vitro and in vivo, indicating that ABI5 directly down-regulated PHO1 expression. Disruption of PHO1 abolished the ABA-insensitive germination phenotypes of abi5 mutant, demonstrating that PHO1 was epistatic to ABI5 . Together, these data demonstrate that PHO1 is involved in ABA-mediated seed germination and early seedling development and transcriptionally regulated by ABI5.